Protein Engineering Strategies for Improved Pharmacokinetics
Sign Up to like & getrecommendations! Published in 2021 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202101633
Abstract: Protein therapeutics have gained momentum in recent years and become a pillar in treating many diseases and the only choice in several ailments. Protein therapeutics are highly specific, tunable, and less toxic than conventional small… read more here.
Keywords: engineering strategies; engineering; half life; strategies improved ... See more keywords
A cocktail of protein engineering strategies: Breaking the enzyme bottleneck one by one for high UTP production in vitro
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28061
Abstract: The pyrimidine metabolic pathway is tightly regulated in microorganisms, allowing limited success in metabolic engineering for the production of pathway‐related substances. Here, we constructed a four‐enzyme coupled system for the in vitro production of uridine… read more here.
Keywords: engineering strategies; kinase; protein engineering; production ... See more keywords
Feruloyl Esterases Protein Engineering to Enhance Their Performance as Biocatalysts: A Review
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200354
Abstract: Feruloyl esterases (FAEs) are versatile enzymes able to release hydroxycinnamic acids or synthesize their ester derivatives, both molecules with interesting biological activities such as: antioxidants, antifungals, antivirals, antifibrotic, anti‐inflammatory, among others. The importance of these… read more here.
Keywords: engineering enhance; feruloyl esterases; performance; protein engineering ... See more keywords
Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1826-4_10
Abstract: Biocatalysis in organic solvents (OSs) is very appealing for the industry in producing bulk and/or fine chemicals, such as pharmaceuticals, biodiesel, and fragrances. The poor performance of enzymes in OSs (e.g., reduced activity, insufficient stability,… read more here.
Keywords: organic solvents; protein engineering; biocatalysis; simulation ... See more keywords
Elastin-like Polymers as Nanovaccines: Protein Engineering of Self-Assembled, Epitope-Exposing Nanoparticles.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2168-4_3
Abstract: In this chapter we describe two unconventional strategies for the formulation of new nanovaccines. Both strategies are based on obtaining chimeric genes that code for proteins in which the major antigens of the pathogens are… read more here.
Keywords: nanovaccines protein; polymers nanovaccines; like polymers; protein engineering ... See more keywords
Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7574-7_8
Abstract: The incorporation of noncanonical amino acids has given protein chemists access to an expanded repertoire of amino acids. This methodology has significantly broadened the scope of protein engineering allowing introduction of amino acids with non-native… read more here.
Keywords: noncanonical amino; amino; amino acids; protein engineering ... See more keywords
Significant improvement of the enantioselectivity of a halohydrin dehalogenase for asymmetric epoxide ring opening reactions by protein engineering
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10356-x
Abstract: Halohydrin dehalogenases (HHDHs) have attracted much attention due to their ability to synthesize enantiomerically enriched epoxides and β-haloalcohols. However, most of the HHDHs exhibit low enantioselectivity. Here, a HHDH from the alphaproteobacteria isolate 46_93_T64 (… read more here.
Keywords: enantioselectivity; protein engineering; significant improvement; halohydrin ... See more keywords
Tailoring Multipurpose Biocatalysts via Protein Engineering Approaches: A Review
Sign Up to like & getrecommendations! Published in 2019 at "Catalysis Letters"
DOI: 10.1007/s10562-019-02821-8
Abstract: The current industrial revolution signifies the high-value of protein engineering. The development of multipurpose biocatalysts is significantly expanding as a result of increased access and enzyme tailoring ability to satisfy the ever-increasing industrial demands. Enzyme-catalyzed… read more here.
Keywords: engineering; engineering approaches; protein engineering; tailoring multipurpose ... See more keywords
Protein engineering for covalent immobilization and enhanced stability through incorporation of multiple noncanonical amino acids
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-017-0127-y
Abstract: In this study, we demonstrate the application of multiple functional properties of proteins generated through coupling of residue-specific and site-specific incorporation method. With green fluorescent protein (GFP) as a model protein, we constructed multifunctional GFP… read more here.
Keywords: incorporation; stability; protein engineering; gfp ... See more keywords
Creation of thermostable l-tryptophan dehydrogenase by protein engineering and its application for l-tryptophan quantification.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.05.010
Abstract: l-Tryptophan dehydrogenase is a new NAD+-dependent amino acid dehydrogenase discovered in Nostoc punctiforme. The enzyme is involved in scytonemin biosynthesis and is highly selective toward l-tryptophan. By a growth-dependent molecular evolution technique, a thermostable mutant… read more here.
Keywords: dehydrogenase; tryptophan dehydrogenase; creation thermostable; thermostable tryptophan ... See more keywords
Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2018.06.002
Abstract: Enzyme immobilization often achieves reusable biocatalysts with improved operational stability and solvent resistance. However, these modifications are generally associated with a decrease in activity or detrimental modifications in catalytic properties. On the other hand, protein… read more here.
Keywords: engineering; enzyme immobilization; immobilization; protein engineering ... See more keywords