Dilution of digestive fish proteases with protein-free blocking reagents prevents loss of catalytic activity during microquantification
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DOI: 10.1007/s12562-020-01422-4
Abstract: The activity of proteases may be lost during microquantification because of their nonspecific binding to tubes and pipette tips. In this study, the effects of blocking reagents (blockers) in dilutions of digestive proteases of yellowtail,… read more here.
Keywords: protein free; blocking reagents; dilution; microquantification ... See more keywords
Green and Tunable Animal Protein-Free Microcarriers for Cell Expansion.
Sign Up to like & getrecommendations! Published in 2020 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.0c16875
Abstract: Cell culture on microcarriers emerges as an alternative of two-dimensional culture to produce large cell doses, which are required for cell-based therapies. Herein, we report a versatile and easy solvent-free greener fabrication process to prepare… read more here.
Keywords: protein free; free microcarriers; animal protein; tunable animal ... See more keywords
Evaluation and use of disaccharides as energy source in protein-free mammalian cell cultures
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DOI: 10.1038/srep45216
Abstract: Mammalian cells are generally considered to be unable to utilize polysaccharides for cell growth because the phospholipid bilayer in the cell membrane has very low permeability to sugars. With the recent discovery of the only… read more here.
Keywords: energy source; protein free; cell; free mammalian ... See more keywords