The TRIM14 PRYSPRY domain mediates protein interaction via its basic interface
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13386
Abstract: Tripartite motif (TRIM)14 was recently shown to be an important molecule against pathogens. Its PRYSPRY domain acts as a protein–protein interaction module. TRIM14 exerts distinct functions via its PRYSPRY domain by interacting with different partners.… read more here.
Keywords: pryspry; trim14 pryspry; protein interaction; pryspry domain ... See more keywords
Labour‐saving construction of a target protein interaction network by selective culture and high‐throughput sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Journal"
DOI: 10.1002/biot.202100204
Abstract: Yeast two‐hybrid (Y2H) is a classic method of screening for protein‐protein interactions. However, the operation process is labor intensive and time consuming, and there is a high possibility of false positives and false negatives. read more here.
Keywords: protein; protein interaction; target protein; construction target ... See more keywords
Optimization of a Class of Dihydrobenzofurane Analogs toward Orally Efficacious YAP‐TEAD Protein‐Protein Interaction Inhibitors
Sign Up to like & getrecommendations! Published in 2023 at "ChemMedChem"
DOI: 10.1002/cmdc.202300051
Abstract: The inhibition of the YAP‐TEAD protein‐protein interaction constitutes a promising therapeutic approach for the treatment of cancers linked to the dysregulation of the Hippo signaling pathway. The identification of a class of small molecules which… read more here.
Keywords: interaction; protein protein; protein interaction; class ... See more keywords
KEAP1‐NRF2 protein–protein interaction inhibitors: Design, pharmacological properties and therapeutic potential
Sign Up to like & getrecommendations! Published in 2022 at "Medicinal Research Reviews"
DOI: 10.1002/med.21925
Abstract: The transcription factor nuclear factor erythroid 2‐related factor 2 (NRF2) is considered the master regulator of the phase II antioxidant response. It controls a plethora of cytoprotective genes related to oxidative stress, inflammation, and protein… read more here.
Keywords: protein protein; protein interaction; keap1 nrf2; pharmacological properties ... See more keywords
Protein‐protein interaction networks as miners of biological discovery
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100190
Abstract: Protein‐protein interactions (PPIs) form the basis of a myriad of biological pathways and mechanism, such as the formation of protein complexes or the components of signaling cascades. Here, we reviewed experimental methods for identifying PPI… read more here.
Keywords: protein protein; protein interaction; biological discovery; interaction networks ... See more keywords
Homo-FRET Imaging to Study Protein-Protein Interaction and Complex Formation in Plants.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1791-5_12
Abstract: Protein-protein interactions in living plant cells can be measured by changes in fluorescence anisotropy due to homo-FRET (Förster Resonance Energy Transfer). Here, the energy transfer between identical fluorophores, e.g., enhanced green fluorescent protein (EGFP) fused… read more here.
Keywords: complex formation; protein protein; homo fret; fret imaging ... See more keywords
Determining the Significance of Protein Network Features and Attributes Using Permutation Testing.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6740-7_15
Abstract: Network analysis methods are increasing in popularity. An approach commonly applied to analyze proteomics data involves the use of protein-protein interaction (PPI) networks to explore the systems-level cooperation between proteins identified in a study. In… read more here.
Keywords: protein; protein interaction; network; significance protein ... See more keywords
LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6747-6_11
Abstract: Protein-protein interactions (PPIs) play an essential role in all biological processes. In vivo, PPIs occur dynamically and depend on extracellular cues. To discover novel protein-protein interactions in mammalian cells, we developed a high-throughput automated technology… read more here.
Keywords: protein; protein interaction; interaction networks; lumier discovery ... See more keywords
Finding RNA-Protein Interaction Sites Using HMMs.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6753-7_13
Abstract: RNA-binding proteins play important roles in the various stages of RNA maturation through binding to its target RNAs. Cross-linking immunoprecipitation coupled with high-throughput sequencing (CLIP-Seq) has made it possible to identify the targeting sites of… read more here.
Keywords: protein; clip seq; protein interaction; rna binding ... See more keywords
Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6940-1_8
Abstract: We recently developed a protein-protein interaction assay, FlimPIA (Firefly luminescent intermediate-based Protein-protein Interaction Assay) based on the catalytic mechanism of firefly luciferase (Fluc) that can be divided into two half-reactions: the adenylation step and the… read more here.
Keywords: protein; protein interaction; firefly; protein protein ... See more keywords
Trimolecular Fluorescence Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in Plants.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9045-0_19
Abstract: RNA-protein interactions play important roles in various eukaryotic biological processes. Molecular imaging of subcellular localization of RNA-protein complexes in plants is critical for understanding these interactions. However, methods to image RNA-protein interactions in living plants… read more here.
Keywords: protein interaction; fluorescence complementation; rna; rna protein ... See more keywords