On the difficulties of characterizing weak protein interactions that are critical for neurotransmitter release
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DOI: 10.1002/2211-5463.13473
Abstract: The mechanism of neurotransmitter release has been extensively characterized, showing that vesicle fusion is mediated by the SNARE complex formed by syntaxin‐1, SNAP‐25 and synaptobrevin. This complex is disassembled by N‐ethylmaleimide sensitive factor (NSF) and… read more here.
Keywords: neurotransmitter; weak interactions; neurotransmitter release; protein interactions ... See more keywords
GST‐IVTT pull‐down: a fast and versatile in vitro method for validating and mapping protein–protein interactions
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DOI: 10.1002/2211-5463.13485
Abstract: Over the past few decades, dozens of in vitro methods have been developed to map, investigate and validate protein–protein interactions. However, most of these approaches are time‐consuming and labour‐intensive or require specialised equipment or substantial amounts… read more here.
Keywords: protein protein; gst ivtt; fast versatile; method ... See more keywords
A simplified direct on‐chip forward or reverse immunoassay for evaluating protein–protein interactions in the serum
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200427
Abstract: The identification of protein–protein interactions is a great challenge. In this study, we fabricated a gold surface‐modified biochip with activated sophorolipids (SLs) in combination with 16‐amino‐1‐hexadecanethiol hydrochloride to detect serum proteins. read more here.
Keywords: protein protein; direct chip; simplified direct; chip forward ... See more keywords
In situ detection of protein interactions for recombinant therapeutic enzymes
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DOI: 10.1002/bit.27621
Abstract: Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly, and posttranslational modifications… read more here.
Keywords: protein; protein interactions; recombinant protein; interactions recombinant ... See more keywords
Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes
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DOI: 10.1002/cbic.201700361
Abstract: Structural and activity studies have revealed the dynamic and transient actions of carrier protein (CP) activity in primary and secondary metabolic pathways. CP‐mediated interactions play a central role in nonribosomal peptide biosynthesis, as they serve… read more here.
Keywords: protein; protein interactions; aryl acid; protein protein ... See more keywords
Multivalent Ligands with Tailor‐Made Anion Binding Motif as Stabilizers of Protein–Protein Interactions
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DOI: 10.1002/cbic.201900288
Abstract: Modulation of protein–protein interactions (PPIs) is essential for understanding and tuning biologically relevant processes. Although inhibitors for PPIs are widely used, the field still lacks the targeted design of stabilizers. Here, we report unnatural stabilizers… read more here.
Keywords: made anion; ligands tailor; protein interactions; multivalent ligands ... See more keywords
Selective Disruption of Survivin's Protein‐Protein Interactions: A Supramolecular Approach Based on Guanidiniocarbonylpyrrole
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DOI: 10.1002/cbic.202100618
Abstract: Targeting specific protein binding sites to interfere with protein‐protein interactions (PPIs) is crucial for the rational modulation of biologically relevant processes. Survivin, which is highly overexpressed in most cancer cells and considered to be a… read more here.
Keywords: protein protein; selective disruption; disruption survivin; protein interactions ... See more keywords
Fragment Screening Yields a Small‐Molecule Stabilizer of 14‐3‐3 Dimers That Modulates Client Protein Interactions
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DOI: 10.1002/cbic.202200178
Abstract: The development of protein‐protein interaction (PPI) inhibitors has been a successful strategy in drug development. However, the identification of PPI stabilizers has proven much more challenging. Here we report a fragment‐based drug screening approach using… read more here.
Keywords: client protein; fragment screening; stabilizer; protein interactions ... See more keywords
Proximity Ligation Assay for Detecting Protein‐Protein Interactions and Protein Modifications in Cells and Tissues in Situ
Sign Up to like & getrecommendations! Published in 2020 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.115
Abstract: Biochemical methods can reveal stable protein‐protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein‐protein interactions in cells and tissues in situ provides important additional… read more here.
Keywords: protein; proximity ligation; protein interactions; ligation assay ... See more keywords
Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.
Sign Up to like & getrecommendations! Published in 2017 at "Current protocols in cell biology"
DOI: 10.1002/cpcb.14
Abstract: We have developed a protocol enabling the study of protein-protein interactions (PPIs) at the proteome level using in vitro-synthesized proteins. Assay preparation requires molecular cloning of the query gene into a vector that supports in… read more here.
Keywords: protein; protein interactions; protein arrays; protein protein ... See more keywords
Mapping oncogenic protein interactions for precision medicine
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DOI: 10.1002/ijc.33954
Abstract: Normal protein‐protein interactions (normPPIs) occur with high fidelity to regulate almost every physiological process. In cancer, this highly organised and precisely regulated network is disrupted, hijacked or reprogrammed resulting in oncogenic protein‐protein interactions (oncoPPIs). OncoPPIs,… read more here.
Keywords: precision medicine; protein; protein interactions; medicine ... See more keywords