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   Articles with "protein interactions" as a keyword



On the difficulties of characterizing weak protein interactions that are critical for neurotransmitter release

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recommendations! Published in 2022 at "FEBS Open Bio"

DOI: 10.1002/2211-5463.13473

Abstract: The mechanism of neurotransmitter release has been extensively characterized, showing that vesicle fusion is mediated by the SNARE complex formed by syntaxin‐1, SNAP‐25 and synaptobrevin. This complex is disassembled by N‐ethylmaleimide sensitive factor (NSF) and… read more here.

Keywords: neurotransmitter; weak interactions; neurotransmitter release; protein interactions ... See more keywords

GST‐IVTT pull‐down: a fast and versatile in vitro method for validating and mapping protein–protein interactions

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recommendations! Published in 2022 at "FEBS Open Bio"

DOI: 10.1002/2211-5463.13485

Abstract: Over the past few decades, dozens of in vitro methods have been developed to map, investigate and validate protein–protein interactions. However, most of these approaches are time‐consuming and labour‐intensive or require specialised equipment or substantial amounts… read more here.

Keywords: protein protein; gst ivtt; fast versatile; method ... See more keywords

Direct Quantification of Protein–Protein Interactions in Living Bacterial Cells

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recommendations! Published in 2025 at "Advanced Science"

DOI: 10.1002/advs.202414777

Abstract: Quantitative measurement of protein–protein interactions (PPIs) within living cells is vital for understanding their cellular functions at the molecular level and for applications in synthetic biology, protein engineering, and drug discovery. Although several techniques have… read more here.

Keywords: living bacterial; protein interactions; biology; bacterial cells ... See more keywords

A simplified direct on‐chip forward or reverse immunoassay for evaluating protein–protein interactions in the serum

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recommendations! Published in 2022 at "Biotechnology Journal"

DOI: 10.1002/biot.202200427

Abstract: The identification of protein–protein interactions is a great challenge. In this study, we fabricated a gold surface‐modified biochip with activated sophorolipids (SLs) in combination with 16‐amino‐1‐hexadecanethiol hydrochloride to detect serum proteins. read more here.

Keywords: protein protein; direct chip; simplified direct; chip forward ... See more keywords
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In situ detection of protein interactions for recombinant therapeutic enzymes

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recommendations! Published in 2020 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.27621

Abstract: Despite their therapeutic potential, many protein drugs remain inaccessible to patients since they are difficult to secrete. Each recombinant protein has unique physicochemical properties and requires different machinery for proper folding, assembly, and posttranslational modifications… read more here.

Keywords: protein; protein interactions; recombinant protein; interactions recombinant ... See more keywords

Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes

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recommendations! Published in 2017 at "ChemBioChem"

DOI: 10.1002/cbic.201700361

Abstract: Structural and activity studies have revealed the dynamic and transient actions of carrier protein (CP) activity in primary and secondary metabolic pathways. CP‐mediated interactions play a central role in nonribosomal peptide biosynthesis, as they serve… read more here.

Keywords: protein; protein interactions; aryl acid; protein protein ... See more keywords
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Multivalent Ligands with Tailor‐Made Anion Binding Motif as Stabilizers of Protein–Protein Interactions

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recommendations! Published in 2019 at "ChemBioChem"

DOI: 10.1002/cbic.201900288

Abstract: Modulation of protein–protein interactions (PPIs) is essential for understanding and tuning biologically relevant processes. Although inhibitors for PPIs are widely used, the field still lacks the targeted design of stabilizers. Here, we report unnatural stabilizers… read more here.

Keywords: made anion; ligands tailor; protein interactions; multivalent ligands ... See more keywords

Selective Disruption of Survivin's Protein‐Protein Interactions: A Supramolecular Approach Based on Guanidiniocarbonylpyrrole

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recommendations! Published in 2022 at "Chembiochem"

DOI: 10.1002/cbic.202100618

Abstract: Targeting specific protein binding sites to interfere with protein‐protein interactions (PPIs) is crucial for the rational modulation of biologically relevant processes. Survivin, which is highly overexpressed in most cancer cells and considered to be a… read more here.

Keywords: protein protein; selective disruption; disruption survivin; protein interactions ... See more keywords

Fragment Screening Yields a Small‐Molecule Stabilizer of 14‐3‐3 Dimers That Modulates Client Protein Interactions

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recommendations! Published in 2022 at "Chembiochem"

DOI: 10.1002/cbic.202200178

Abstract: The development of protein‐protein interaction (PPI) inhibitors has been a successful strategy in drug development. However, the identification of PPI stabilizers has proven much more challenging. Here we report a fragment‐based drug screening approach using… read more here.

Keywords: client protein; fragment screening; stabilizer; protein interactions ... See more keywords

14‐3‐3 Protein‐Protein Interactions: From Mechanistic Understanding to Their Small‐Molecule Stabilization

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recommendations! Published in 2024 at "ChemBioChem"

DOI: 10.1002/cbic.202400214

Abstract: Protein‐protein interactions (PPIs) are of utmost importance for maintenance of cellular homeostasis. Herein, a central role can be found for 14‐3‐3 proteins. These hub‐proteins are known to bind hundreds of interaction partners, thereby regulating their… read more here.

Keywords: protein interactions; role; stabilization; protein ... See more keywords

Proximity Ligation Assay for Detecting Protein‐Protein Interactions and Protein Modifications in Cells and Tissues in Situ

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recommendations! Published in 2020 at "Current Protocols in Cell Biology"

DOI: 10.1002/cpcb.115

Abstract: Biochemical methods can reveal stable protein‐protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein‐protein interactions in cells and tissues in situ provides important additional… read more here.

Keywords: protein; proximity ligation; protein interactions; ligation assay ... See more keywords