Covalent Protein Labeling at Glutamic Acids.
Sign Up to like & getrecommendations! Published in 2017 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2017.03.015
Abstract: Covalent labeling of amino acids in proteins by reactive small molecules, in particular at cysteine SH and lysine NH groups, is a powerful approach to identify and characterize proteins and their functions. However, for the… read more here.
Keywords: covalent protein; protein labeling; covalent; biology ... See more keywords
Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.2c00411
Abstract: Staphylococcus aureus sortase A is a transpeptidase that has been extensively exploited for site-specific modification of proteins and was originally used to attach a labeling reagent containing an LPXTG recognition sequence to a protein or… read more here.
Keywords: protein labeling; depsipeptide substrates; sortase; combined application ... See more keywords
Improved Photoinduced Fluorogenic Alkene-Tetrazole Reaction for Protein Labeling.
Sign Up to like & getrecommendations! Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00562
Abstract: The 1,3-dipolar cycloaddition reaction between an alkene and a tetrazole represents one elegant and rare example of fluorophore-forming bioorthogonal chemistry. This is an attractive reaction for imaging applications in live cells that requires less intensive… read more here.
Keywords: tetrazole reaction; protein labeling; alkene tetrazole; chemistry ... See more keywords
Fluorogenic Protein Labeling Probes to Study the Morphological Interplay between PreLamin and Mature Lamin.
Sign Up to like & getrecommendations! Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00611
Abstract: Although many protein labeling probes have been developed to elucidate the trafficking and turnover processes of cell surface proteins, real-time tracking of intracellular proteins remains a challenging task. Herein, we describe a new design to… read more here.
Keywords: mature; labeling probes; prelamin mature; protein labeling ... See more keywords
Light-Controlled Traceless Protein Labeling via Decaging Thio-o-naphthoquinone Methide Chemistry.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c02742
Abstract: We report the molecular design of a novel multifunctional reagent and its application for light-controlled selective protein labeling. This molecule integrates functions of protein-ligand recognition, bioconjugation, ligand cleavage, and photoactivation by merging the photochemistries of… read more here.
Keywords: naphthoquinone methide; light controlled; chemistry; protein labeling ... See more keywords
Protein-Labeling Fluorescent Probe Reveals Ectodomain Shedding of Transmembrane Carbonic Anhydrases.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00679
Abstract: Ectodomain shedding is a form of limited proteolysis in which a protease cleaves a transmembrane protein, releasing the extracellular domain from the cell surface. Cells use this process to regulate a wide variety of biological… read more here.
Keywords: protein labeling; ectodomain shedding; carbonic anhydrases; cell surface ... See more keywords
Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00740
Abstract: Chemical proteomics is a powerful technology that can be used in the studies of the functions of uncharacterized proteins in the human proteome. It relies on a suitable bioconjugation strategy for protein labeling. This could… read more here.
Keywords: protein labeling; bioconjugation protein; induced bioconjugation; bioconjugation ... See more keywords
Site-Specific Protein Labeling and Generation of Defined Ubiquitin-Protein Conjugates Using an Asparaginyl Endopeptidase
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c02191
Abstract: Asparaginyl endopeptidases (AEPs) have recently been widely utilized for peptide and protein modification. Labeling is however restricted to protein termini, severely limiting flexibility and scope in creating diverse conjugates as needed for therapeutic and diagnostic… read more here.
Keywords: specific protein; protein labeling; generation; site specific ... See more keywords
Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b05098
Abstract: N-Hydroxysuccinimide (NHS)-esters are widely used to label proteins nonselectively on free amino groups. Such broad labeling can be disadvantageous because it can interfere with protein structure or function and because stoichiometry is poorly controlled. Here… read more here.
Keywords: protein; site specific; protein labeling; ubiquitin ligase ... See more keywords
Improved split fluorescent proteins for endogenous protein labeling
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-00494-8
Abstract: Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell–cell contact. To expand this toolset, we have developed a screening strategy for the direct… read more here.
Keywords: split fluorescent; protein labeling; fluorescent proteins; endogenous protein ... See more keywords
Fluorogenic protein labeling using a genetically encoded unstrained alkene† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc03635j Click here for additional data file.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical Science"
DOI: 10.1039/c6sc03635j
Abstract: A new fluorogenic bioorthogonal reaction between styrene (an unstrained alkene) and a tetrazine was developed. read more here.
Keywords: protein labeling; labeling using; fluorogenic protein; unstrained alkene ... See more keywords