IL-35 Detection in B Cells at the mRNA and Protein Level.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1237-8_8
Abstract: Emerging research suggests that IL-35-producing regulatory B cells accumulate in patients and mouse models of pancreatic cancer, one of the most lethal cancers, characterized by late diagnosis, high mortality, and morbidity. Identification of IL-35-producing B… read more here.
Keywords: cells mrna; detection; mrna protein; regulatory cells ... See more keywords
Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.01.035
Abstract: To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous genome-wide measurements of mRNA, translation, and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of… read more here.
Keywords: protein; isoform; coordinated protein; pervasive coordinated ... See more keywords
An efficient and assumption-free method to approximate protein level distribution in the two-states gene expression model.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of theoretical biology"
DOI: 10.1016/j.jtbi.2017.08.019
Abstract: Stochastic fluctuations at each step of gene expression might influence protein levels distributions across cell populations. However, current methods to model protein distribution of intrinsic gene expression dynamics are either computationally inefficient or rely on… read more here.
Keywords: gene; distribution; gene expression; protein level ... See more keywords
Quantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.0c00464
Abstract: Labeling approaches using isobaric chemical tags (e.g., isobaric tagging for relative and absolute quantification, iTRAQ and tandem mass tag, TMT) have been widely applied for the quantification of peptides and proteins in bottom-up MS. However,… read more here.
Keywords: mass tag; mass; protein level; complex samples ... See more keywords
Assessing average somatic CAG repeat instability at the protein level
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-55202-x
Abstract: Sandwich ELISA-based methods use Abs that target the expanded polyglutamine (polyQ) tract to quantify mutant huntingtin (mHTT). Using Meso Scale Discovery (MSD) assay, the mHTT signal detected with MW1 Ab correlated with polyQ length and… read more here.
Keywords: length; cag repeat; repeat instability; protein level ... See more keywords
Lentiviral mediated RPE65 gene transfer in healthy hiPSCs-derived retinal pigment epithelial cells markedly increased RPE65 mRNA, but modestly protein level
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-65657-y
Abstract: The retinal pigment epithelium (RPE) is a monolayer of cobblestone-like epithelial cells that accomplishes critical functions for the retina. Several protocols have been published to differentiate pluripotent stem cells into RPE cells suitable for disease… read more here.
Keywords: epithelial cells; rpe65 mrna; protein level; retinal pigment ... See more keywords
Arginyltransferase (Ate1) regulates the RGS7 protein level and the sensitivity of light-evoked ON-bipolar responses
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-88628-3
Abstract: Regulator of G-protein signaling 7 (RGS7) is predominately present in the nervous system and is essential for neuronal signaling involving G-proteins. Prior studies in cultured cells showed that RGS7 is regulated via proteasomal degradation, however… read more here.
Keywords: regulates rgs7; ate1 regulates; rgs7 protein; protein level ... See more keywords
Evaluation of machine learning models on protein level inference from prioritized RNA features
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac091
Abstract: The parallel measurement of transcriptome and proteome revealed unmatched profiles. Since proteomic analysis is more expensive and challenging than transcriptomic analysis, the question of how to use messenger RNA (mRNA) expression data to predict protein… read more here.
Keywords: protein level; rna; machine learning; expression ... See more keywords
Effects of reduced protein level and dietary amino acid supplementation on growth, body composition and intestinal morphometry of silver catfish ( Rhamdia quelen )
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture Research"
DOI: 10.1111/are.14830
Abstract: The reduction in the protein level and the supplementation of lysine (Lys) and methionine (Met) in the diet were evaluated in the performance and intestinal morphometry of silver catfish (Rhamdia quelen). The study was used… read more here.
Keywords: morphometry silver; silver catfish; intestinal morphometry; catfish rhamdia ... See more keywords
The pyruvate kinase (PK) to hexokinase enzyme activity ratio and erythrocyte PK protein level in the diagnosis and phenotype of PK deficiency
Sign Up to like & getrecommendations! Published in 2020 at "British Journal of Haematology"
DOI: 10.1111/bjh.16724
Abstract: Diagnosis of pyruvate kinase deficiency (PKD), the most common cause of hereditary non‐spherocytic haemolytic anaemia, remains challenging in routine practice and no biomarkers for clinical severity have been characterised. This prospective study enrolled 41 patients… read more here.
Keywords: pyruvate kinase; enzyme activity; protein level; enzyme ... See more keywords
Proteome Expression and Survival Strategies of a Proteorhodopsin-Containing Vibrio Strain under Carbon and Nitrogen Limitation
Sign Up to like & getrecommendations! Published in 2022 at "mSystems"
DOI: 10.1128/msystems.01263-21
Abstract: Understanding the nutrient stress responses of proteorhodopsin-bearing microbes like Vibrio campbellii yields insights into microbial contributions to nutrient cycling, lifestyles of emerging pathogens in aquatic environments, and protein-level adaptations implemented during times of nutrient limitation.… read more here.
Keywords: protein level; carbon; limitation; survival ... See more keywords