Articles with "protein loading" as a keyword



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Preparation, functionalization and characterization of rice husk silica for lipase immobilization via adsorption.

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Published in 2019 at "Enzyme and microbial technology"

DOI: 10.1016/j.enzmictec.2019.05.001

Abstract: Silica has been extracted from rice husks via a simple hydrothermal process and functionalized with triethoxy(octyl)silane -OCTES (Octyl-SiO2) and (3-aminopropyl)triethoxysilane - 3-APTES (Amino-SiO2), with the aim of using it as support to immobilize lipase from… read more here.

Keywords: octyl sio2; adsorption; via adsorption; protein loading ... See more keywords
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ACTB and GAPDH appear at multiple SDS-PAGE positions, thus not suitable as reference genes for determining protein loading in techniques like Western blotting

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Published in 2021 at "Open Life Sciences"

DOI: 10.1515/biol-2021-0130

Abstract: Abstract We performed polyacrylamide gel electrophoresis of human proteins with sodium dodecyl sulfate, isolated proteins at multiple positions, and then used liquid chromatography and tandem mass spectrometry (LC-MS/MS) to determine the protein identities. Although beta-actin… read more here.

Keywords: reference genes; determining protein; protein; genes determining ... See more keywords