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Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200209
Abstract: To mimic the levels of spatiotemporal control that exist in nature, tools for chemically induced dimerization (CID) are employed to manipulate proteināprotein interactions. Although linker composition is known to influence speed and efficiency of heterobifunctional…
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Keywords:
phase separation;
protein localization;
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Published in 2018 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927618007341
Abstract: Bridging light and electron microscopy through correlative light and electron microscopy (CLEM) enables imaging of protein localization within a detailed subcellular context [1]. Certain CLEM approaches, such as array tomography, nanofEM, are compatible with super-resolution…
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Keywords:
microscopy;
protein localization;
correlative light;
electron microscopy ... See more keywords
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Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-26041-z
Abstract: The GO-Cellular Component (GO-CC) ontology provides a controlled vocabulary for the consistent description of the subcellular compartments or macromolecular complexes where proteins may act. Current machine learning-based methods used for the automated GO-CC annotation of…
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Keywords:
annotation;
fgga classifiers;
protein localization;
prediction ... See more keywords
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Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac719
Abstract: Motivation While multi-channel fluorescence microscopy is a vital imaging method in biological studies, the number of channels that can be imaged simultaneously is limited by technical and hardware limitations such as emission spectra cross-talk. One…
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Keywords:
localization;
gan digitally;
protein localization;
4dr gan ... See more keywords
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Published in 2022 at "Cold Spring Harbor perspectives in biology"
DOI: 10.1101/cshperspect.a041249
Abstract: High-fidelity protein localization is essential to define the identities and functions of different organelles and to maintain cellular homeostasis. Accurate localization of nascent proteins requires specific protein targeting pathways as well as quality control (QC)…
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Keywords:
endoplasmic reticulum;
protein localization;
localization;
fidelity ... See more keywords
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Published in 2022 at "Plant biotechnology journal"
DOI: 10.1111/pbi.13790
Abstract: Fluorescent tagging protein localization (FTPL) and bimolecular fluorescence complementation (BiFC) are popular tools for in vivo analyses of the subcellular localizations of proteins and protein-protein interactions in plant cells. The efficiency of fluorescent fusion protein…
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Keywords:
vector toolbox;
protein localization;
fusion protein;
fluorescent fusion ... See more keywords
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Published in 2021 at "Biomolecules"
DOI: 10.3390/biom11020264
Abstract: Identifying localization of proteins and their specific subpopulations associated with certain cellular compartments is crucial for understanding protein function and interactions with other macromolecules. Fluorescence microscopy is a powerful method to assess protein localizations, with…
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Keywords:
protein;
classification;
network;
protein localization ... See more keywords
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Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23010281
Abstract: Fidelity of protein targeting is essential for the proper biogenesis and functioning of organelles. Unlike replication, transcription and translation processes, in which multiple mechanisms to recognize and reject noncognate substrates are established in energetic and…
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Keywords:
protein;
protein localization;
fidelity cotranslational;
protein targeting ... See more keywords