Influence of media composition on recombinant monoclonal IgA1 glycosylation analysed by lectin-based protein microarray and MALDI-MS
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biotechnology"
DOI: 10.1016/j.jbiotec.2020.03.009
Abstract: Abstract Glycosylation of therapeutic glycoproteins significantly affects their physico-chemical properties, bioactivity and immunogenicity. The determination of glycan composition is highly important regarding their development and production. Therefore, there is a demand for analytical techniques enabling… read more here.
Keywords: protein microarray; based protein; lectin based; iga1 ... See more keywords
Analysis of swine antigen-specific antibody responses to Mycoplasma hyopneumoniae infection determined by protein microarray.
Sign Up to like & getrecommendations! Published in 2019 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2019.02.010
Abstract: Pigs harbor several different species of mycoplasmas, of which Mycoplasma hyopneumoniae presents the most significant economic impact on the swine industry. While ELISAs are the predominant diagnostic assay to measure antibody responses during infection with… read more here.
Keywords: protein microarray; infection; antibody; antigen specific ... See more keywords
Fabrication of a protein microarray by fluorous-fluorous interactions
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-07571-4
Abstract: Fluorous-modified surfaces have emerged as a powerful tool for the immobilization of fluorous-tagged biomolecules based on their specificity and the strength of fluorous-fluorous interactions. To fabricate a fluorous-based protein microarray, we designed two strategies for… read more here.
Keywords: fabrication protein; fluorous fluorous; protein microarray; fluorous interactions ... See more keywords
Rapid selection of aptamers based on protein microarray
Sign Up to like & getrecommendations! Published in 2019 at "RSC Advances"
DOI: 10.1039/c8ra09232j
Abstract: We report a novel method for the efficient screening of aptamers from a complex ssDNA library based on a microarray chip, which was named Microarray-SELEX. read more here.
Keywords: rapid selection; based protein; selection aptamers; protein microarray ... See more keywords
Controlling the immobilization process of an optically enhanced protein microarray for highly reproducible immunoassay.
Sign Up to like & getrecommendations! Published in 2021 at "Nanoscale"
DOI: 10.1039/d0nr08407g
Abstract: By virtue of its high throughput multiplex detection capability, superior read-out sensitivity, and tiny analyte consumption, an optically enhanced protein microarray assay has been developed as a promising diagnostic tool for various applications, ranging from… read more here.
Keywords: controlling immobilization; protein microarray; optically enhanced; enhanced protein ... See more keywords
SARS-CoV-2 multi-antigen protein microarray for detailed characterization of antibody responses in COVID-19 patients
Sign Up to like & getrecommendations! Published in 2022 at "PLOS ONE"
DOI: 10.1101/2022.10.14.512324
Abstract: Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) target multiple epitopes on different domains of the spike protein, and other SARS-CoV-2 proteins. We developed a SARS-CoV-2 multi-antigen protein microarray with the nucleocapsid, spike and… read more here.
Keywords: covid patients; antigen protein; cov multi; multi antigen ... See more keywords
Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Veterinary Internal Medicine"
DOI: 10.1111/jvim.16600
Abstract: Abstract Background The diagnostic value of allergen‐specific immunoglobulin E (IgE) in horses with asthma is uncertain. A recently developed protein microarray detected abnormally high latex‐specific IgE concentrations in the serum of horses with severe asthma.… read more here.
Keywords: horses asthma; balf; serum horses; microarray ... See more keywords
PAWER: protein array web exploreR
Sign Up to like & getrecommendations! Published in 2020 at "BMC Bioinformatics"
DOI: 10.1186/s12859-020-03722-z
Abstract: Background Protein microarray is a well-established approach for characterizing activity levels of thousands of proteins in a parallel manner. Analysis of protein microarray data is complex and time-consuming, while existing solutions are either outdated or… read more here.
Keywords: protein; protein microarray; analysis; protein array ... See more keywords
Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs
Sign Up to like & getrecommendations! Published in 2019 at "PLoS ONE"
DOI: 10.1371/journal.pone.0217290
Abstract: In order to monitor the occurrence of zoonotic agents in pig herds as well as to improve herd health management, the development of new cost-effective diagnostic methods for pigs is necessary. In this study, a… read more here.
Keywords: diseases pigs; production diseases; protein microarray; zoonotic agents ... See more keywords
KILchip v1.0: A Novel Plasmodium falciparum Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2018.02866
Abstract: Passive transfer studies in humans clearly demonstrated the protective role of IgG antibodies against malaria. Identifying the precise parasite antigens that mediate immunity is essential for vaccine design, but has proved difficult. Completion of the… read more here.
Keywords: protein microarray; prioritization; falciparum; plasmodium falciparum ... See more keywords