Tracking carrier protein motions with Raman spectroscopy
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10184-2
Abstract: Engineering microbial biosynthetic pathways represents a compelling route to gain access to expanded chemical diversity. Carrier proteins (CPs) play a central role in biosynthesis, but the fast motions of CPs make their conformational dynamics difficult… read more here.
Keywords: carrier protein; carrier; tracking carrier; protein motions ... See more keywords
Temporal and spatial resolution of distal protein motions that activate hydrogen tunneling in soybean lipoxygenase.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2211630120
Abstract: The enzyme soybean lipoxygenase (SLO) provides a prototype for deep tunneling mechanisms in hydrogen transfer catalysis. This work combines room temperature X-ray studies with extended hydrogen-deuterium exchange experiments to define a catalytically-linked, radiating cone of… read more here.
Keywords: distal protein; hydrogen; protein motions; temporal spatial ... See more keywords