Control Intracellular Protein Condensates with Light.
Sign Up to like & getrecommendations! Published in 2024 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.4c00305
Abstract: Protein phase transitions are gaining traction among biologists for their wide-ranging roles in biological regulation. However, achieving precise control over these phenomena in vivo remains a formidable task. Optogenetic techniques present us with a potential… read more here.
Keywords: control; protein phase; biology; phase transitions ... See more keywords
Vitamin C Drives Reentrant Actin Phase Transition: Biphasic Exocytosis Regulation Revealed by Single-Vesicle Electrochemistry.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.4c02710
Abstract: Unveiling molecular mechanisms that dominate protein phase dynamics has been a pressing need for deciphering the intricate intracellular modulation machinery. While ions and biomacromolecules have been widely recognized for modulating protein phase separations, effects of… read more here.
Keywords: protein phase; electrochemistry; single vesicle; drives reentrant ... See more keywords
Aggregation or phase separation can be induced in highly charged proteins by small charged biomolecules.
Sign Up to like & getrecommendations! Published in 2022 at "Soft matter"
DOI: 10.1039/d2sm00384h
Abstract: Protein phase separation in biological systems has captured the attention of scientists in the last decade; however, the main mechanism underlying protein phase separation in cells remains unclear. Biologists, physicists, and chemists have all tried… read more here.
Keywords: protein phase; highly charged; charged biomolecules; separation ... See more keywords
Sequence determinants of protein phase separation and recognition by protein phase-separated condensates through molecular dynamics and active learning.
Sign Up to like & getrecommendations! Published in 2024 at "Faraday discussions"
DOI: 10.1039/d4fd00099d
Abstract: Elucidating how protein sequence determines the properties of disordered proteins and their phase-separated condensates is a great challenge in computational chemistry, biology, and biophysics. Quantitative molecular dynamics simulations and derived free energy values can in… read more here.
Keywords: protein phase; protein; phase separated; sequence ... See more keywords
DAmFRET measures saturating concentrations and toxicities of protein phase transitions in vivo.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular biology of the cell"
DOI: 10.1091/mbc.e22-11-0503
Abstract: Protein phase transitions broadly govern protein function and dysfunction. However, analyzing the consequences of specific phase transitions in cells is hindered by the low throughput and limited resolution of fluorescence microscopy, and this problem is… read more here.
Keywords: measures saturating; phase transitions; saturating concentrations; concentrations toxicities ... See more keywords
Leveraging a large language model to predict protein phase transition: A physical, multiscale, and interpretable approach
Sign Up to like & getrecommendations! Published in 2024 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1101/2023.11.21.568125
Abstract: Protein phase transitions (PPTs) from the soluble state to a dense liquid phase (forming droplets via liquid-liquid phase separation) or to solid aggregates (such as amyloids) play key roles in pathological processes associated with age-related… read more here.
Keywords: large language; protein phase; language; model ... See more keywords
Protein phase separation: A novel therapy for cancer?
Sign Up to like & getrecommendations! Published in 2020 at "British Journal of Pharmacology"
DOI: 10.1111/bph.15242
Abstract: In recent years, phase separation has been increasingly reported to play a pivotal role in a wide range of biological processes. Due to the close relationships between cancer and disorders in intracellular physiological function, the… read more here.
Keywords: phase separation; therapy; protein phase; separation ... See more keywords
An Interpretable Machine-Learning Algorithm to Predict Disordered Protein Phase Separation Based on Biophysical Interactions
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12081131
Abstract: Protein phase separation is increasingly understood to be an important mechanism of biological organization and biomaterial formation. Intrinsically disordered protein regions (IDRs) are often significant drivers of protein phase separation. A number of protein phase… read more here.
Keywords: biophysical interactions; phase separation; protein phase; disordered protein ... See more keywords
Protein Phase Separation: New Insights into Carcinogenesis
Sign Up to like & getrecommendations! Published in 2022 at "Cancers"
DOI: 10.3390/cancers14235971
Abstract: Simple Summary The global burden of cancer is substantial and growing. Among 22 groups of diseases and injuries in the GBD 2019 study, cancer was second only to cardiovascular diseases for the number of deaths… read more here.
Keywords: phase separation; separation new; protein phase; separation ... See more keywords
Protein phase separation: new insights into cell division.
Sign Up to like & getrecommendations! Published in 2023 at "Acta biochimica et biophysica Sinica"
DOI: 10.3724/abbs.2023093
Abstract: As the foundation for the development of multicellular organisms and the self-renewal of single cells, cell division is a highly organized event which segregates cellular components into two daughter cells equally or unequally, thus producing… read more here.
Keywords: division; cell division; phase separation; protein phase ... See more keywords