A simplified LC−MS/MS method for rapid determination of cycloserine in small-volume human plasma using protein precipitation coupled with dilution techniques to overcome matrix effects and its application to a pharmacokinetic study
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0249-2
Abstract: Matrix effects have been a major concern when developing LC−MS/MS methods for quantitative bioanalysis of cycloserine. Sample handling procedures including solid phase extraction or derivatization have been reported previously by researchers to overcome matrix effects… read more here.
Keywords: protein precipitation; dilution; study; matrix effects ... See more keywords
Rapid and quantitative protein precipitation for proteome analysis by mass spectrometry.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00867
Abstract: Protein precipitation is a common front-end preparation strategy for proteome analysis, as well as other applications (e.g. protein depletion for small molecule analysis, bulk commercial preparation of protein). Highly variable conditions used to precipitate proteins,… read more here.
Keywords: recovery; protein precipitation; precipitation; analysis ... See more keywords
Highly effective identification of drug targets at the proteome level by pH-dependent protein precipitation
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d2sc03326g
Abstract: Fully understanding the target spaces of drugs is essential for investigating the mechanism of drug action and side effects, as well as for drug discovery and repurposing. In this study, we present an energetics-based approach,… read more here.
Keywords: protein precipitation; identification; dependent protein; target proteins ... See more keywords
Distribution of Protein Precipitation Capacity within Variable Proanthocyanidin Fingerprints
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DOI: 10.3390/molecules25215002
Abstract: Proanthocyanidins (PAs) are highly bioactive plant specialized metabolites. One of their most characteristic features is their ability to precipitate proteins. In this study, eleven plant species were used to study the structure–activity patterns between PAs… read more here.
Keywords: precipitation capacity; plant species; protein precipitation; plant ... See more keywords