YBac: A novel and simple baculovirus system for heterologous protein production in Spodoptera frugiperda 9 (Sf9) cells.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202200558
Abstract: The baculovirus expression vector system (BEVS) has been widely used for heterologous protein expression due to its powerful functionality and easy access to commercial expression vectors. Currently, most laboratories prefer two strategies for protein production… read more here.
Keywords: system; protein production; production; heterologous protein ... See more keywords
Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells
Sign Up to like & getrecommendations! Published in 2022 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202100124
Abstract: Mammalian cells are commonly used to produce recombinant protein therapeutics, but suffer from a high cost per mg of protein produced. There is therefore great interest in improving protein yields to reduce production cost. We… read more here.
Keywords: ribosomal protein; protein production; rpl10 r98s; production ... See more keywords
High-Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads.
Sign Up to like & getrecommendations! Published in 2020 at "Small"
DOI: 10.1002/smll.202005523
Abstract: Efficient production hosts are a key requirement for bringing biopharmaceutical and biotechnological innovations to the market. In this work, a truly universal high-throughput platform for optimization of microbial protein production is described. Using droplet microfluidics,… read more here.
Keywords: protein; production; protein production; gel beads ... See more keywords
Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris
Sign Up to like & getrecommendations! Published in 2018 at "Yeast"
DOI: 10.1002/yea.3301
Abstract: Pichia pastoris expression system has been widely used in recombinant protein production. So far the majority of heterologous proteins are expressed by methanol inducible promoter PAOX1 and constitutive promoter PGAP. The use of other promoters… read more here.
Keywords: protein production; pichia pastoris; recombinant protein; promoter ... See more keywords
Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_2
Abstract: Despite the importance of recombinant protein production in the academy and industrial fields, many issues concerning the expression of soluble and homogeneous products are still unsolved. Several strategies were developed to overcome these obstacles; however,… read more here.
Keywords: solubility problem; coli; recombinant protein; protein production ... See more keywords
Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8730-6_14
Abstract: With an increasing number of blockbuster drugs being recombinant mammalian proteins, protein production platforms that focus on mammalian proteins have had a profound impact in many areas of basic and applied research. Many groups, both… read more here.
Keywords: genes involved; production; protein production; throughput rnai ... See more keywords
Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9024-5_12
Abstract: Approximately 30% of the genes in the human genome code for membrane proteins, and yet we know relatively little about these complex molecules. Therefore, the biochemical and structural characterization of this challenging class of proteins… read more here.
Keywords: production; protein production; cholesterol; membrane ... See more keywords
High-Throughput Protein Production in Yeast.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_4
Abstract: Yeasts are versatile single-celled fungi that grow to high cell densities on inexpensive media. With well-studied genetics and metabolism and a wealth of knowledge available about their propagation and growth in academic as well as… read more here.
Keywords: throughput; protein production; throughput protein; production yeast ... See more keywords
A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_5
Abstract: Recombinant protein expression and purification is an essential component of biomedical research and drug discovery. Advances in automation and laboratory robotics have enabled the development of highly parallel and rapid processes for cell culture and… read more here.
Keywords: protein; transient stable; production; protein production ... See more keywords
Enhanced Recombinant Protein Production of Soluble, Highly Active and Immobilizable PNGase F
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biotechnology"
DOI: 10.1007/s12033-022-00464-6
Abstract: High resolution analysis of N-glycans can be performed after their endoglycosidase mediated removal from proteins. N-glycosidase F peptide (PNGase F) is one the most frequently used enzyme for this purpose. Because of the significant demand… read more here.
Keywords: pngase; recombinant protein; protein production; production ... See more keywords
Impact of oxidative stress on protein production by Bordetella pertussis for vaccine production
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2019.107359
Abstract: Abstract The response of Bordetella pertussis to induced oxidative stress during fermentation was assessed. B. pertussis is the causal agent of whooping cough in humans and purified pertactin (PRN) is used as one of the… read more here.
Keywords: stress; oxidative stress; bordetella pertussis; protein production ... See more keywords