mem-iLID, a fast and economic protein purification method
Sign Up to like & getrecommendations! Published in 2021 at "Bioscience Reports"
DOI: 10.1042/bsr20210800
Abstract: Abstract Protein purification is the vital basis to study the function, structure and interaction of proteins. Widely used methods are affinity chromatography-based purifications, which require different chromatography columns and harsh conditions, such as acidic pH… read more here.
Keywords: purification; method; protein purification; mem ilid ... See more keywords
Metal-chelated magnetic nanoparticles for protein C purification
Sign Up to like & getrecommendations! Published in 2019 at "Separation Science and Technology"
DOI: 10.1080/01496395.2019.1618327
Abstract: ABSTRACT In the study, magnetic poly(hydroxyethylmethacrylate-N-methacryloyl-(L)-histidine) (mPHEMAH) nanoparticles were prepared by miniemulsion polymerisation using N-methacryloyl-(L)-histidinemethylester as basic monomer. The specific surface area of the magnetic nanoparticles was found to be 1050 m2/g. Maximum Protein C… read more here.
Keywords: metal chelated; chelated magnetic; mphemah; magnetic nanoparticles ... See more keywords
Resource allocation strategies for protein purification operations
Sign Up to like & getrecommendations! Published in 2020 at "IISE Transactions"
DOI: 10.1080/24725854.2019.1680908
Abstract: Abstract We analyze resource allocation challenges in protein purification operations where differences in scientist capabilities can lead to significantly different outcomes. We use queuing models to capture the underlying dynamics and quantify the performance of… read more here.
Keywords: resource allocation; allocation strategies; purification operations; protein purification ... See more keywords
Application of nanofibrous protein for the purification of contaminated water as a next generational sorption technology: a review
Sign Up to like & getrecommendations! Published in 2023 at "Nanotechnology"
DOI: 10.1088/1361-6528/acbd9f
Abstract: Globally, wastes from agricultural and industrial activities cause water pollution. Pollutants such as microbes, pesticides, and heavy metals in contaminated water bodies beyond their threshold limits result in several diseases like mutagenicity, cancer, gastrointestinal problems,… read more here.
Keywords: nanofibrils protein; purification; purification contaminated; protein purification ... See more keywords
Protein A and Protein G Purification of Antibodies.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099143
Abstract: Protein A and Protein G are immunoglobulin-binding proteins expressed in Staphylococcus aureus and Streptococcus sp., respectively, that have been adapted for use in purifying large amounts of IgG. They are available covalently attached to affinity… read more here.
Keywords: protein; purification antibodies; affinity; protein protein ... See more keywords
Protein purification strategies must consider downstream applications and individual biological characteristics
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Cell Factories"
DOI: 10.1186/s12934-022-01778-5
Abstract: Background Proteins are used as reagents in a broad range of scientific fields. The reliability and reproducibility of experimental data will largely depend on the quality of the (recombinant) proteins and, consequently, these should undergo… read more here.
Keywords: downstream applications; purification strategies; characteristics protein; protein purification ... See more keywords
1Progress, applications, challenges and prospects of protein purification technology
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.1028691
Abstract: Protein is one of the most important biological macromolecules in life, which plays a vital role in cell growth, development, movement, heredity, reproduction and other life activities. High quality isolation and purification is an essential… read more here.
Keywords: purification; applications challenges; protein purification; life ... See more keywords
Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.849542
Abstract: There are several commercial chromatographic systems for protein purification; however, development of cost-effective 3H-grade (high yield, high purity, and high activity) purification approaches is highly demanded. Here, we establish a methodology for encapsulating the IM7-displaying… read more here.
Keywords: purification; im7 displaying; protein purification; displaying yeast ... See more keywords