Protein Quantification and Imaging by Surface‐Enhanced Raman Spectroscopy and Similarity Analysis
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.201903638
Abstract: Abstract Protein quantification techniques such as immunoassays have been improved considerably, but they have several limitations, including time‐consuming procedures, low sensitivity, and extrinsic detection. Because direct surface‐enhanced Raman spectroscopy (SERS) can detect intrinsic signals of… read more here.
Keywords: protein; surface enhanced; enhanced raman; protein quantification ... See more keywords
Development and evaluation of new methods for protein quantification in dissolving microneedles formulations.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113453
Abstract: Dissolving microneedles (DMNs) are vital for the transdermal delivery of protein-based drugs due to their painless penetration, effective drug permeation, and safety characteristics. Thus, it is necessary to quantify the proteins encapsulated inside the DMNs.… read more here.
Keywords: quantification; dissolving microneedles; bca assay; protein quantification ... See more keywords
Peptide Selection for Accurate Targeted Protein Quantification via a Dimethylation High-Resolution Mass Spectrum Strategy with a Peptide Release Kinetic Model
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DOI: 10.1021/acsomega.9b02002
Abstract: A crucial step in accurate targeted protein quantification using targeted proteomics is to determine optimal proteotypic peptides representing targeted proteins. In this study, a workflow of peptide selection to determine proteotypic peptides using a dimethylation… read more here.
Keywords: peptide selection; protein quantification; targeted protein; quantification ... See more keywords
Artificial Intelligence Understands Peptide Observability and Assists With Absolute Protein Quantification
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2018.01559
Abstract: Targeted mass spectrometry has become the method of choice to gain absolute quantification information of high quality, which is essential for a quantitative understanding of biological systems. However, the design of absolute protein quantification assays… read more here.
Keywords: peptide observability; protein quantification; absolute protein; quantification ... See more keywords
A Point-of-Care Device for Fully Automated, Fast and Sensitive Protein Quantification via qPCR
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12070537
Abstract: This paper presents a fully automated point-of-care device for protein quantification using short-DNA aptamers, where no manual sample preparation is needed. The device is based on our novel aptamer-based methodology combined with real-time polymerase chain… read more here.
Keywords: point care; quantification; care device; device ... See more keywords