Reconstitution of an Anti‐HER2 Antibody Paratope by Grafting Dual CDR‐Derived Peptides onto a Small Protein Scaffold
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Journal"
DOI: 10.1002/biot.202000078
Abstract: Target‐binding small proteins are promising alternatives to conventional monoclonal antibodies (mAbs), offering advantages in terms of tissue penetration and manufacturing costs. Recently, a design strategy to create small proteins called fluctuation‐regulated affinity proteins (FLAPs) consisting… read more here.
Keywords: protein scaffold; dual cdr; cdr derived; cdr ... See more keywords
Functionalization of a symmetric protein scaffold: Redundant folding nuclei and alternative oligomeric folding pathways
Sign Up to like & getrecommendations! Published in 2022 at "Protein Science"
DOI: 10.1002/pro.4301
Abstract: Successful de novo protein design ideally targets specific folding kinetics, stability thermodynamics, and biochemical functionality, and the simultaneous achievement of all these criteria in a single step design is challenging. Protein design is potentially simplified… read more here.
Keywords: folding nuclei; redundant folding; design; protein scaffold ... See more keywords
Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines.
Sign Up to like & getrecommendations! Published in 2021 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2021.06.001
Abstract: Green fluorescent protein (GFP) is a widely used scaffold for protein-based targeted nanomedicines because of its high biocompatibility, biological neutrality and outstanding structural stability. However, being immunogenicity a major concern in the development of drug… read more here.
Keywords: protein; protein scaffold; gfp like; like protein ... See more keywords
Multifaceted Bioanalytical Methods for the Comprehensive Pharmacokinetic and Catabolic Assessment of MEDI3726, an Anti-PSMA PBD Antibody-Drug Conjugate.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c01187
Abstract: Complex biotherapeutic modalities such as antibody-drug conjugates (ADC), present significant challenges for the comprehensive bioanalytical characterization of their pharmacokinetics (PK) and catabolism in both pre-clinical and clinical settings. Thus, the bioanalytical strategy for ADCs must… read more here.
Keywords: protein scaffold; bioanalytical methods; multifaceted bioanalytical; drug ... See more keywords
Metal-Assisted Assembly of Protein Containers Loaded with Inorganic Nanoparticles.
Sign Up to like & getrecommendations! Published in 2018 at "Inorganic chemistry"
DOI: 10.1021/acs.inorgchem.8b01995
Abstract: Protein containers are suitable building blocks for bioinorganic materials. Here, we show that high concentrations of magnesium ions induce the formation of a unitary protein scaffold, whereas low magnesium concentration leads to a binary protein… read more here.
Keywords: protein scaffold; protein containers; nanoparticles metal; inorganic nanoparticles ... See more keywords
New Disulphide Bond in Cystatin-Based Protein Scaffold Prevents Domain-Swap-Mediated Oligomerization and Stabilizes the Functionally Active Form
Sign Up to like & getrecommendations! Published in 2019 at "ACS Omega"
DOI: 10.1021/acsomega.9b02269
Abstract: Peptide aptamers built using engineered scaffolds are a valuable alternative to monoclonal antibodies in many research applications because of their smaller size, versatility, specificity for chosen targets, and ease of production. However, inserting peptides needed… read more here.
Keywords: protein scaffold; based protein; disulphide bond; bond ... See more keywords
Protein-Scaffold Directed Nanoscale Assembly of T Cell Ligands: Artificial Antigen Presentation with Defined Valency, Density, and Ratio.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00119
Abstract: Tuning antigen presentation to T cells is a critical step in investigating key aspects of T cell activation. However, existing technologies have a limited ability to control the spatial and stoichiometric organization of T cell… read more here.
Keywords: protein scaffold; cell ligands; scaffold directed; antigen presentation ... See more keywords
The protein scaffold calibrates metal specificity and activation in MerR sensors
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14151
Abstract: MerR metalloregulators are the central components of many biosensor platforms designed to report metal contamination. However, most MerR proteins are non‐specific. This makes it difficult to apply these biosensors in the analysis of real environmental… read more here.
Keywords: calibrates metal; scaffold calibrates; metal specificity; protein scaffold ... See more keywords
Vaccine Efficacy of Self-Assembled Multimeric Protein Scaffold Particles Displaying the Glycoprotein Gn Head Domain of Rift Valley Fever Virus
Sign Up to like & getrecommendations! Published in 2021 at "Vaccines"
DOI: 10.3390/vaccines9030301
Abstract: Compared to free antigens, antigens immobilized on scaffolds, such as nanoparticles, generally show improved immunogenicity. Conventionally, antigens are conjugated to scaffolds through genetic fusion or chemical conjugation, which may result in impaired assembly or heterogeneous… read more here.
Keywords: protein scaffold; rift valley; head domain; multimeric protein ... See more keywords