Next-generation protein sequencing and individual ion mass spectrometry enable complementary analysis of interleukin-6
Sign Up to like & getrecommendations! Published in 2025 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-025-06120-7
Abstract: The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, we evaluated the complementarity of two cutting-edge proteomic technologies—single-molecule protein sequencing and… read more here.
Keywords: ion mass; mass spectrometry; individual ion; protein sequencing ... See more keywords
Strategies for Development of a Next-Generation Protein Sequencing Platform.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2019.09.005
Abstract: Proteomic analysis can be a critical bottleneck in cellular characterization. The current paradigm relies primarily on mass spectrometry of peptides and affinity reagents (i.e., antibodies), both of which require a priori knowledge of the sample.… read more here.
Keywords: protein; generation protein; strategies development; protein sequencing ... See more keywords
Highly Robust de Novo Full-Length Protein Sequencing.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03718
Abstract: Accurate full-length sequencing of a purified unknown protein is still challenging nowadays due to the error-prone mass-spectrometry (MS)-based methods. De novo identified peptide sequence largely contain errors, undermining the accuracy of assembly. Bias on the… read more here.
Keywords: full length; length protein; accuracy; protein sequencing ... See more keywords
Enhancing De Novo Protein Sequencing through the C-Terminal Labeling Strategy: Resolving Isobaric Ambiguities by Electron-Transfer/Higher Energy Collision Dissociation (EThcD).
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c03459
Abstract: De novo protein sequencing via a bottom-up approach requires various proteases to produce overlapping peptides. However, peptides generated by proteases other than trypsin, LysC, and ArgC often yield C-terminal fragments with suboptimal ionization in positive… read more here.
Keywords: labeling strategy; protein sequencing; protein; novo protein ... See more keywords
Machine Learning Prediction of the Transmission Function for Protein Sequencing with Graphene Nanoslit.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c13405
Abstract: Protein sequencing has rapidly changed the landscape of healthcare and life science by accelerating the growth of diagnostics and personalized medicines for a variety of fatal diseases. Next-generation nanopore/nanoslit sequencing is promising to achieve single-molecule… read more here.
Keywords: protein sequencing; transmission function; prediction; prediction transmission ... See more keywords
Learning dynamical information from static protein and sequencing data
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-13307-x
Abstract: Many complex processes, from protein folding to neuronal network dynamics, can be described as stochastic exploration of a high-dimensional energy landscape. Although efficient algorithms for cluster detection in high-dimensional spaces have been developed over the… read more here.
Keywords: high dimensional; network; learning dynamical; time ... See more keywords
Nanopore sensors for single molecular protein detection: Research progress based on computer simulations
Sign Up to like & getrecommendations! Published in 2023 at "IET Nanobiotechnology"
DOI: 10.1049/nbt2.12124
Abstract: Abstract As biological macromolecules, proteins are involved in important cellular functions ranging from DNA replication and biosynthesis to metabolic signalling and environmental sensing. Protein sequencing can help understand the relationship between protein function and structure,… read more here.
Keywords: protein sequencing; computer simulations; detection; nanopore sensors ... See more keywords
The AI revolution comes to protein sequencing.
Sign Up to like & getrecommendations! Published in 2025 at "Science"
DOI: 10.1126/science.ady0877
Abstract: By identifying unknown proteins, new systems could aid research in many areas. read more here.
Keywords: protein sequencing; comes protein; revolution comes;