Affinities and Kinetics Detection of Protein-Small Molecule Interactions with a Monolayer MoS2 -Based Optical Imaging Platform.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202202622
Abstract: Quantifying the binding kinetics and affinities of protein-small molecule interactions is critical for biomarker validation, drug discovery, and deep understanding of various biological processes at the molecular-scale. Novel approaches are demanded as most common label-free… read more here.
Keywords: small molecule; binding kinetics; molecule; molecule interactions ... See more keywords
Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1394-8_12
Abstract: This chapter outlines a protocol developed to prepare a purified deuterated membrane protein for a small-angle neutron scattering (SANS) experiment. SANS is a noninvasive technique well suited to studying membrane protein solution structures, and deuteration… read more here.
Keywords: protein; small angle; deuterated membrane; membrane protein ... See more keywords
Protein–small molecule docking with receptor flexibility in iMOLSDOCK
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Computer-Aided Molecular Design"
DOI: 10.1007/s10822-018-0152-8
Abstract: We have earlier reported the iMOLSDOCK technique to perform ‘induced-fit’ peptide–protein docking. iMOLSDOCK uses the mutually orthogonal Latin squares (MOLSs) technique to sample the conformation and the docking pose of the small molecule ligand and… read more here.
Keywords: receptor; protein small; small molecule; imolsdock ... See more keywords
RosettaLigandEnsemble: A Small-Molecule Ensemble-Driven Docking Approach
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DOI: 10.1021/acsomega.7b02059
Abstract: RosettaLigand is a protein–small-molecule (ligand) docking software capable of predicting binding poses and is used for virtual screening of medium-sized ligand libraries. Structurally similar small molecules are generally found to bind in the same pose… read more here.
Keywords: molecule; molecule ensemble; small molecule; rosettaligandensemble small ... See more keywords
Protein-induced fluorescence enhancement for a simple and universal detection of protein/small molecule interactions
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DOI: 10.1039/c8ra08515c
Abstract: We herein describe a novel and efficient method for the detection of protein/small molecule (SM) interactions, which relies on the protein-induced fluorescence enhancement (PIFE). In this method, a duplex probe is designed to position Cy3… read more here.
Keywords: protein induced; small molecule; induced fluorescence; protein small ... See more keywords
In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20020378
Abstract: Nuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need… read more here.
Keywords: cell nmr; small molecule; protein small; molecule interactions ... See more keywords