DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, Burkholderia dolosa PC543: effect of N‐/C‐terminal His Tag extension on protein solubility and activity
Sign Up to like & getrecommendations! Published in 2018 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201800036
Abstract: NAD(P)+ dependent formate dehydrogenase (FDH) is an oxidoreductase used as a biocatalyst to regenerate NAD(P)H in reductase‐mediated chiral synthesis reactions. Solvent stability and the need to reduce NADP+ to NADPH, due to the high cost… read more here.
Keywords: solubility activity; dmso tolerant; enzyme; solubility ... See more keywords
Quantitative Evaluation of Protein Solubility in Aqueous Solutions by PEG-Induced Liquid-Liquid Phase Separation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9678-0_3
Abstract: This chapter describes an experimental method to quantitatively evaluate the solubility of proteins in aqueous solutions. Measurement of protein solubility can be challenging because low solubility can be manifested through various pathways (e.g., crystallization, aggregation,… read more here.
Keywords: liquid; aqueous solutions; solubility; liquid liquid ... See more keywords
Solubility and aggregation behavior of protein fractions from the heterotrophically cultivated microalga Chlorella protothecoides.
Sign Up to like & getrecommendations! Published in 2019 at "Food research international"
DOI: 10.1016/j.foodres.2018.08.037
Abstract: Protein solubility in water is a key property of food proteins. The aim of this work was to study the solubility and microstructural properties as a function of pH of both protein fractions (water-soluble (WSPE)… read more here.
Keywords: protein; chlorella protothecoides; solubility; protein fractions ... See more keywords
Impact of Germination on the Protein Solubility and Antinutritive Compounds of Lupinus angustifolius and Vicia faba in the Production of Protein-Rich Legume-Based Beverages.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.3c01249
Abstract: Lupines and faba beans are protein-rich legumes, which can be utilized as a plant-based substitute for animal proteins in human nutrition in general and in the beverage industry in particular. However, their application is hampered… read more here.
Keywords: faba beans; protein solubility; germination; protein rich ... See more keywords
Development of a Method for Fast Assessment of Protein Solubility Based on Ultrasonic Dispersion and Differential Centrifugation Technology
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DOI: 10.1021/acsomega.2c03666
Abstract: Protein solubility is very important for protein crystallization, bioprocess development, and protein application. In this study, a method based on the stability of a protein dispersion system is proposed for fast assessment of protein solubility,… read more here.
Keywords: dispersion; solubility; protein solubility; fast assessment ... See more keywords
Codon usage clusters correlation: towards protein solubility prediction in heterologous expression systems in E. coli
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DOI: 10.1038/s41598-018-29035-z
Abstract: Production of soluble recombinant proteins is crucial to the development of industry and basic research. However, the aggregation due to the incorrect folding of the nascent polypeptides is still a mayor bottleneck. Understanding the factors… read more here.
Keywords: codon usage; correlation; expression; protein solubility ... See more keywords
Generation of a Library of Carbohydrate-Active Enzymes for Plant Biomass Deconstruction
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DOI: 10.3390/ijms23074024
Abstract: In nature, the deconstruction of plant carbohydrates is carried out by carbohydrate-active enzymes (CAZymes). A high-throughput (HTP) strategy was used to isolate and clone 1476 genes obtained from a diverse library of recombinant CAZymes covering… read more here.
Keywords: active enzymes; protein solubility; carbohydrate active; plant ... See more keywords
Promotion of Protein Solubility and Reduction in Stiffness in Human Lenses by Aggrelyte-1: Implications for Reversing Presbyopia
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24032196
Abstract: With aging, human lenses lose the ability to focus on nearby objects due to decreases in accommodative ability, a condition known as presbyopia. An increase in stiffness or decrease in lens elasticity due to protein… read more here.
Keywords: reduction; stiffness; protein solubility; human lenses ... See more keywords