Allosteric influence of extremophile hairpin motif mutations on the protein splicing activity of a hyperthermophilic intein.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00348
Abstract: Protein splicing is a post-translational process mediated by an intein, whereby the intein excises itself from a precursor protein with concomitant ligation of the flanking two polypeptides. The intein that interrupts the DNA Polymerase II… read more here.
Keywords: protein splicing; motif; extremophile hairpin; intein ... See more keywords
Conditional Alternative Protein Splicing Promoted by Inteins from Haloquadratum walsbyi.
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DOI: 10.1021/acs.biochem.1c00788
Abstract: Protein splicing is a post-translational process by which an intervening protein, or an intein, catalyzes its own excision from flanking polypeptides, or exteins, coupled to extein ligation. Four inteins interrupt the MCM helicase of the… read more here.
Keywords: protein splicing; haloquadratum walsbyi; conditional alternative; alternative protein ... See more keywords
A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.2210/pdb6frg/pdb
Abstract: Inteins remove themselves from a precursor protein by protein splicing. Due to the concomitant structural changes of the host protein, this self-processing reaction has enabled many applications in protein biotechnology and chemical biology. We show… read more here.
Keywords: intein; block histidine; extein; protein splicing ... See more keywords
The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms
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DOI: 10.3390/ijms21218367
Abstract: Protein splicing catalyzed by inteins utilizes many different combinations of amino-acid types at active sites. Inteins have been classified into three classes based on their characteristic sequences. We investigated the structural basis of the protein… read more here.
Keywords: class; class intein; hedgehog intein; protein splicing ... See more keywords