Notch signaling increases PPARγ protein stability and enhances lipid uptake through AKT in IL‐4‐stimulated THP‐1 and primary human macrophages
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12858
Abstract: Notch signaling and nuclear receptor PPARγ are involved in macrophage polarization, but cross talk between them has not been reported in macrophages. In this study, the effect of Notch signaling on PPARγ in IL‐4‐stimulated human… read more here.
Keywords: ppar; akt; ppar protein; protein stability ... See more keywords
Foldable Detergents for Membrane Protein Stability
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200276
Abstract: Detergents are widely used for membrane protein structural study. Many recently developed detergents contain multiple tail and head groups, which are typically connected via a small and branched linker. Due to their inherent compact structures,… read more here.
Keywords: detergents membrane; alkyl chain; protein stability; membrane protein ... See more keywords
Predicting protein stability and solubility changes upon mutations: data perspective
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DOI: 10.1002/cctc.202000933
Abstract: Understanding mutational effects on protein stability and solubility is of particular importance for creating industrially relevant biocatalysts, resolving mechanisms of many human diseases, and producing efficient biopharmaceuticals, to name a few. For in silico predictions,… read more here.
Keywords: predicting protein; protein stability; stability solubility; solubility changes ... See more keywords
The use of pharmacological chaperones in rare diseases caused by reduced protein stability
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202200222
Abstract: Rare diseases are most often caused by inherited genetic disorders that, after translation, will result in a protein with altered function. Decreased protein stability is the most frequent mechanism associated with a congenital pathogenic missense… read more here.
Keywords: use pharmacological; pharmacological chaperones; protein stability; stability ... See more keywords
Effects of aging on protein expression in mice brain microvessels: ROS scavengers, mRNA/protein stability, glycolytic enzymes, mitochondrial complexes, and basement membrane components.
Sign Up to like & getrecommendations! Published in 2021 at "GeroScience"
DOI: 10.1007/s11357-021-00468-1
Abstract: Differentially expressed (DE) proteins in the cortical microvessels (MVs) of young, middle-aged, and old male and female mice were evaluated using discovery-based proteomics analysis (> 4,200 quantified proteins/group). Most DE proteins (> 90%) showed no significant differences… read more here.
Keywords: old mice; mrna protein; basement membrane; protein stability ... See more keywords
Identifying critical process steps to protein stability during spray drying using a vibrating mesh or a two‐fluid nozzle
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Pharmaceutical Sciences"
DOI: 10.1016/j.ejps.2018.11.027
Abstract: &NA; The aim of this study was to identify critical steps to protein stability during spray drying using two different nozzle types: a vibrating mesh nozzle and a standard two‐fluid nozzle in a Büchi B‐90… read more here.
Keywords: fluid nozzle; spray; spray drying; protein stability ... See more keywords
Influenza Hemagglutinin Protein Stability, Activation, and Pandemic Risk.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in microbiology"
DOI: 10.1016/j.tim.2018.03.005
Abstract: For decades, hemagglutinin (HA) protein structure and its refolding mechanism have served as a paradigm for understanding protein-mediated membrane fusion. HA trimers are in a high-energy state and are functionally activated by low pH. Over… read more here.
Keywords: protein; stability activation; influenza hemagglutinin; hemagglutinin protein ... See more keywords
The Influence of Arginine and Counter-Ions: Antibody Stability during Freeze-Drying.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2020.12.009
Abstract: Amino acids, for example L-arginine, are used in lyophilisation as crystalline bulking, buffering, viscosity reducing or stabilising excipients. In this study, arginine was formulated with different counter ions (hydrochloride, citrate, lactobionate, phosphate, and succinate). A… read more here.
Keywords: antibody; arginine; protein stability; lactobionate ... See more keywords
Improved Repeat Protein Stability by Combined Consensus and Computational Protein Design.
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DOI: 10.1021/acs.biochem.2c00083
Abstract: High protein stability is an important feature for proteins used as therapeutics, as diagnostics, and in basic research. We have previously employed consensus design to engineer optimized Armadillo repeat proteins (ArmRPs) for sequence-specific recognition of… read more here.
Keywords: consensus; repeat; cap; protein stability ... See more keywords
Three Simple Properties Explain Protein Stability Change upon Mutation
Sign Up to like & getrecommendations! Published in 2021 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.1c00201
Abstract: Accurate prediction of protein stability upon mutation enables rational engineering of new proteins and insights into protein evolution and monogenetic diseases caused by single-point amino acid substitutions. Many tools have been developed to this aim,… read more here.
Keywords: amino acid; protein stability; model; stability ... See more keywords
Toward Compilation of Balanced Protein Stability Data Sets: Flattening the ΔΔG Curve through Systematic Enrichment
Sign Up to like & getrecommendations! Published in 2022 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.2c00054
Abstract: Often studies analyzing stability data sets and/or predictors ignore neutral mutations and use a binary classification scheme labeling only destabilizing and stabilizing mutations. Recognizing that highly concentrated neutral mutations interfere with data set quality, we… read more here.
Keywords: protein stability; concentrated neutral; stability data; neutral mutations ... See more keywords