Notch signaling increases PPARγ protein stability and enhances lipid uptake through AKT in IL‐4‐stimulated THP‐1 and primary human macrophages
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12858
Abstract: Notch signaling and nuclear receptor PPARγ are involved in macrophage polarization, but cross talk between them has not been reported in macrophages. In this study, the effect of Notch signaling on PPARγ in IL‐4‐stimulated human… read more here.
Keywords: ppar; akt; ppar protein; protein stability ... See more keywords
EZH2 Inhibition Enhances PD‐L1 Protein Stability Through USP22‐Mediated Deubiquitination in Colorectal Cancer
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DOI: 10.1002/advs.202308045
Abstract: The regulation of PD‐L1 is the key question, which largely determines the outcome of the immune checkpoint inhibitors (ICIs) based therapy. However, besides the transcription level, the protein stability of PD‐L1 is closely correlated with… read more here.
Keywords: cancer; protein stability; inhibition enhances; ezh2 inhibition ... See more keywords
Foldable Detergents for Membrane Protein Stability
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200276
Abstract: Detergents are widely used for membrane protein structural study. Many recently developed detergents contain multiple tail and head groups, which are typically connected via a small and branched linker. Due to their inherent compact structures,… read more here.
Keywords: detergents membrane; alkyl chain; protein stability; membrane protein ... See more keywords
Predicting protein stability and solubility changes upon mutations: data perspective
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DOI: 10.1002/cctc.202000933
Abstract: Understanding mutational effects on protein stability and solubility is of particular importance for creating industrially relevant biocatalysts, resolving mechanisms of many human diseases, and producing efficient biopharmaceuticals, to name a few. For in silico predictions,… read more here.
Keywords: predicting protein; protein stability; stability solubility; solubility changes ... See more keywords
The use of pharmacological chaperones in rare diseases caused by reduced protein stability
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202200222
Abstract: Rare diseases are most often caused by inherited genetic disorders that, after translation, will result in a protein with altered function. Decreased protein stability is the most frequent mechanism associated with a congenital pathogenic missense… read more here.
Keywords: use pharmacological; pharmacological chaperones; protein stability; stability ... See more keywords
An intein‐based biosensor to measure protein stability in vivo
Sign Up to like & getrecommendations! Published in 2024 at "Protein Science"
DOI: 10.1002/pro.4925
Abstract: Biosensors to measure protein stability in vivo are valuable tools for a variety of applications. Previous work has demonstrated that a tripartite design, whereby a protein of interest (POI) is inserted within a reporter, can… read more here.
Keywords: stability vivo; biosensor; stability; measure protein ... See more keywords
Protein stability models fail to capture epistatic interactions of double point mutations
Sign Up to like & getrecommendations! Published in 2024 at "Protein Science"
DOI: 10.1002/pro.70003
Abstract: There is strong interest in accurate methods for predicting changes in protein stability resulting from amino acid mutations to the protein sequence. Recombinant proteins must often be stabilized to be used as therapeutics or reagents,… read more here.
Keywords: double point; stability; stability models; protein stability ... See more keywords
Effects of aging on protein expression in mice brain microvessels: ROS scavengers, mRNA/protein stability, glycolytic enzymes, mitochondrial complexes, and basement membrane components.
Sign Up to like & getrecommendations! Published in 2021 at "GeroScience"
DOI: 10.1007/s11357-021-00468-1
Abstract: Differentially expressed (DE) proteins in the cortical microvessels (MVs) of young, middle-aged, and old male and female mice were evaluated using discovery-based proteomics analysis (> 4,200 quantified proteins/group). Most DE proteins (> 90%) showed no significant differences… read more here.
Keywords: old mice; mrna protein; basement membrane; protein stability ... See more keywords
Identifying critical process steps to protein stability during spray drying using a vibrating mesh or a two‐fluid nozzle
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Pharmaceutical Sciences"
DOI: 10.1016/j.ejps.2018.11.027
Abstract: &NA; The aim of this study was to identify critical steps to protein stability during spray drying using two different nozzle types: a vibrating mesh nozzle and a standard two‐fluid nozzle in a Büchi B‐90… read more here.
Keywords: fluid nozzle; spray; spray drying; protein stability ... See more keywords
Influenza Hemagglutinin Protein Stability, Activation, and Pandemic Risk.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in microbiology"
DOI: 10.1016/j.tim.2018.03.005
Abstract: For decades, hemagglutinin (HA) protein structure and its refolding mechanism have served as a paradigm for understanding protein-mediated membrane fusion. HA trimers are in a high-energy state and are functionally activated by low pH. Over… read more here.
Keywords: protein; stability activation; influenza hemagglutinin; hemagglutinin protein ... See more keywords
The Influence of Arginine and Counter-Ions: Antibody Stability during Freeze-Drying.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2020.12.009
Abstract: Amino acids, for example L-arginine, are used in lyophilisation as crystalline bulking, buffering, viscosity reducing or stabilising excipients. In this study, arginine was formulated with different counter ions (hydrochloride, citrate, lactobionate, phosphate, and succinate). A… read more here.
Keywords: antibody; arginine; protein stability; lactobionate ... See more keywords