Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis
Sign Up to like & getrecommendations! Published in 2022 at "PLOS ONE"
DOI: 10.1371/journal.pone.0278972
Abstract: Chlamydomonas reinhardtii is an important model organism for the study of many cellular processes, and protein tagging is an increasingly indispensable tool for these studies. To circumvent the disadvantages of conventional approaches in creating a… read more here.
Keywords: crispr cas9; protein tagging; situ; chlamydomonas reinhardtii ... See more keywords
Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach
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DOI: 10.7554/elife.75050
Abstract: The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins by homology-directed repair in a variety of species. Despite its revolutionary success, there remains an urgent need for increased simplicity and efficiency of… read more here.
Keywords: cas9; endogenous protein; protein tagging; crispr cas9 ... See more keywords