In-Depth Analysis of C Terminomes Based on LysC Digestion and Site-Selective Dimethylation.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b05338
Abstract: Analysis of protein C termini is very important for functional annotations of proteomes, while proteome-wide C termini analysis still poses substantial challenges. Here we described a simple and robust strategy for specific isolation of protein… read more here.
Keywords: lysc digestion; analysis; protein termini; termini ... See more keywords
Basic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b05280
Abstract: Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical/enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS) based protein C-termini analysis approaches have been established.… read more here.
Keywords: protein; cation exchange; protein termini; chemistry ... See more keywords
NAPT, an unbiased approach for sequential analysis of the protein N- and C-terminome.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc03778e
Abstract: Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the Protein N- and C-Terminome (NAPT)… read more here.
Keywords: terminome napt; protein terminome; analysis protein; protein termini ... See more keywords