Protein translocation in mitochondria: Sorting out the Toms, Tims, Pams, Sams and Mia.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS letters"
DOI: 10.1002/1873-3468.14614
Abstract: Mitochondria contain 902 (yeast) to 1.136 (mouse, humans) verified proteins. Except for a very small number of mitochondrially encoded core components of the respiratory chain, mitochondrial proteins are encoded by nuclear genes and synthesized in… read more here.
Keywords: protein translocation; sorting toms; import; mitochondrial import ... See more keywords
Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein‐translocation channel
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27306
Abstract: In the initial step of sugar metabolism, sugar‐specific transporters play a decisive role in the passage of sugars through plasma membranes into cytoplasm. The SecY complex (SecYEG) in bacteria forms a membrane channel responsible for… read more here.
Keywords: secy; transport; channel; protein translocation ... See more keywords
Investigating protein translocation in the presence of an electrolyte concentration gradient across a solid‐state nanopore
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202100346
Abstract: Electrolyte chemistry plays an important role in the transport properties of analytes through nanopores. Here, we report the translocation properties of the protein human serum transferrin (hSTf) in asymmetric LiCl salt concentrations with either positive… read more here.
Keywords: investigating protein; concentration; protein translocation; chemical gradients ... See more keywords
Neddylation promotes protein translocation between the cytoplasm and nucleus.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.07.012
Abstract: Neddylation is an ubiquitin-like modification of proteins that affects the activity, stability and protein-protein interaction of its substrates. Apart from its role as a promoter for Cullin ring E3 ligase to positively regulate the ubiquitylation… read more here.
Keywords: cytoplasm nucleus; promotes protein; neddylation; protein translocation ... See more keywords
Remote Coupled Drastic β-Barrel to β-Sheet Transition of the Protein Translocation Motor.
Sign Up to like & getrecommendations! Published in 2018 at "Structure"
DOI: 10.1016/j.str.2018.01.002
Abstract: The membrane protein SecDF, belonging to the RND superfamily, enhances protein translocation at the extracytoplasmic side using a proton gradient. Here, we report the crystal structure of SecDF in a form we named Super-membrane-facing (Super F) form, demonstrating… read more here.
Keywords: protein; remote coupled; transition; protein translocation ... See more keywords
Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-40682-8
Abstract: Anaplasma phagocytophilum, the aetiologic agent of human granulocytic anaplasmosis (HGA) is an obligate intracellular Gram-negative bacterium with the genome size of 1.47 megabases. The intracellular life style and small size of genome suggest that A.… read more here.
Keywords: anaplasma phagocytophilum; identification; protein translocation; translocation assay ... See more keywords
Human apo-SRP72 and SRP68/72 complex structures reveal the molecular basis of protein translocation
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Molecular Cell Biology"
DOI: 10.1093/jmcb/mjx010
Abstract: Abstract The co-translational targeting or insertion of secretory and membrane proteins into the endoplasmic reticulum (ER) is a key biological process mediated by the signal recognition particle (SRP). In eukaryotes, the SRP68–SRP72 (SRP68/72) heterodimer plays… read more here.
Keywords: srp68; srp72 srp68; srp72; apo srp72 ... See more keywords
Mechanism of Protein Translocation by the Sec61 Translocon Complex.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor perspectives in biology"
DOI: 10.1101/cshperspect.a041250
Abstract: The endoplasmic reticulum (ER) is a major site for protein synthesis, folding, and maturation in eukaryotic cells, responsible for production of secretory proteins and most integral membrane proteins. The universally conserved protein-conducting channel Sec61 complex… read more here.
Keywords: translocation sec61; sec61 translocon; protein translocation; mechanism protein ... See more keywords
SecA-Mediated Protein Translocation through the SecYEG Channel.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiology spectrum"
DOI: 10.1128/microbiolspec.psib-0028-2019
Abstract: In bacteria, the Sec translocase mediates the translocation of proteins into and across the cytoplasmic membrane. It consists of a protein conducting channel SecYEG, the ATP-dependent motor SecA, and the accessory SecDF complex. Here we… read more here.
Keywords: translocation secyeg; mediated protein; translocation; protein translocation ... See more keywords
Structural and Mechanistic Insights into Protein Translocation.
Sign Up to like & getrecommendations! Published in 2017 at "Annual review of cell and developmental biology"
DOI: 10.1146/annurev-cellbio-100616-060439
Abstract: Many proteins are translocated across the endoplasmic reticulum (ER) membrane in eukaryotes or the plasma membrane in prokaryotes. These proteins use hydrophobic signal sequences or transmembrane (TM) segments to trigger their translocation through the protein-conducting… read more here.
Keywords: structural mechanistic; protein translocation; translocation; insights protein ... See more keywords
Clearing Traffic Jams During Protein Translocation Across Membranes
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2020.610689
Abstract: Protein translocation across membranes is a critical facet of protein biogenesis in compartmentalized cells as proteins synthesized in the cytoplasm often need to traverse across lipid bilayers via proteinaceous channels to reach their final destinations.… read more here.
Keywords: across membranes; clearing traffic; translocation across; protein translocation ... See more keywords