An out‐of‐equilibrium definition of protein turnover
Sign Up to like & getrecommendations! Published in 2023 at "BioEssays"
DOI: 10.1002/bies.202200209
Abstract: Protein turnover (PT) has been formally defined only in equilibrium conditions, which is ill‐suited to quantify PT during dynamic processes that occur during embryogenesis or (extra) cellular signaling. In this Hypothesis, we propose a definition… read more here.
Keywords: equilibrium; equilibrium definition; protein turnover;
Generalizing the definition of protein turnover to out‐of‐equilibrium conditions
Sign Up to like & getrecommendations! Published in 2023 at "BioEssays"
DOI: 10.1002/bies.202300059
Abstract: Protein turnover (PT) refers to the continuous process of protein syn-thesis and degradation in living organisms. Proteins are one of the majordeterminantsofcellularactivitiesanddamagedproteinsneedto be replaced to maintain an efficient proteome, making PT essential for the performance… read more here.
Keywords: turnover equilibrium; definition protein; protein turnover; generalizing definition ... See more keywords
Site‐Specific Protein Labeling with Fluorophores as a Tool To Monitor Protein Turnover
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.201900651
Abstract: Proteins that terminally fail to acquire their native structure are detected and degraded by cellular quality control systems. Insights into cellular protein quality control are key to a better understanding of how cells establish and… read more here.
Keywords: protein; protein turnover; site specific; monitor protein ... See more keywords
Accumulation of “Old Proteins” and the Critical Need for MS‐based Protein Turnover Measurements in Aging and Longevity
Sign Up to like & getrecommendations! Published in 2019 at "Proteomics"
DOI: 10.1002/pmic.201800403
Abstract: Aging and age‐related diseases are accompanied by proteome remodeling and progressive declines in cellular machinery required to maintain protein homeostasis (proteostasis), such as autophagy, ubiquitin‐mediated degradation, and protein synthesis. While many studies have focused on… read more here.
Keywords: protein; protein turnover; accumulation old; proteins critical ... See more keywords
Modelling of protein turnover provides insight for metabolic demands on those specific amino acids utilised at disproportionately faster rates than other amino acids
Sign Up to like & getrecommendations! Published in 2019 at "Amino Acids"
DOI: 10.1007/s00726-019-02734-1
Abstract: The nitrogen balance is regulated by factors such as diet, physical activity, age, pathogenic challenges, and climatic conditions. A paradigm was developed from published recommended rates of protein intake (g/kg/day) with corresponding rates of endogenous… read more here.
Keywords: protein turnover; amino acid; amino acids; turnover ... See more keywords
Presynaptic activity and protein turnover are correlated at the single-synapse level.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.108841
Abstract: Synaptic transmission relies on the continual exocytosis and recycling of synaptic vesicles. Aged vesicle proteins are prevented from recycling and are eventually degraded. This implies that active synapses would lose vesicles and vesicle-associated proteins over… read more here.
Keywords: protein turnover; single synapse; turnover; synapse level ... See more keywords
TurnoveR: A Skyline External Tool for Analysis of Protein Turnover in Metabolic Labeling Studies.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00173
Abstract: In spite of its central role in biology and disease, protein turnover is a largely understudied aspect of most proteomic studies due to the complexity of computational workflows that analyze in vivo turnover rates. To… read more here.
Keywords: metabolic labeling; turnover rates; analysis; protein turnover ... See more keywords
Efficiency of Protein Renewal Is Limited by Feed Intake and Not by Protein Lifetime in Aging Caenorhabditis elegans.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00383
Abstract: Protein turnover maintains the proteome's functional integrity. Here, protein turnover efficiency over time in wild-type Caenorhabditis elegans was assessed using inverse [15N]-pulse labeling up to 7 days after the egg-laying phase at 20 °C. Isotopic… read more here.
Keywords: time; caenorhabditis elegans; protein renewal; protein turnover ... See more keywords
Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-23272-z
Abstract: The association between reduced myofilament force-generating capacity (Fmax) and heart failure (HF) is clear, however the underlying molecular mechanisms are poorly understood. Here, we show impaired Fmax arises from reduced BAG3-mediated sarcomere turnover. Myofilament BAG3… read more here.
Keywords: protein turnover; bag3 mediated; heart failure;
Using protein turnover to expand the applications of transcriptomics
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DOI: 10.1038/s41598-021-83886-7
Abstract: RNA expression and protein abundance are often at odds when measured in parallel, raising questions about the functional implications of transcriptomics data. Here, we present the concept of persistence, which attempts to address this challenge… read more here.
Keywords: protein; protein turnover; turnover expand; protein abundance ... See more keywords
DeuteRater: a tool for quantifying peptide isotope precision and kinetic proteomics
Sign Up to like & getrecommendations! Published in 2017 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btx009
Abstract: Motivation: Using mass spectrometry to measure the concentration and turnover of the individual proteins in a proteome, enables the calculation of individual synthesis and degradation rates for each protein. Software to analyze concentration is readily… read more here.
Keywords: protein turnover; isotope; turnover; deuterater tool ... See more keywords