Effect of Subtilisin-like Proteinase of Bacillus pumilus 3–19 on Pseudomonas aeruginosa Biofilms
Sign Up to like & getrecommendations! Published in 2019 at "BioNanoScience"
DOI: 10.1007/s12668-019-00617-z
Abstract: Pseudomonas aeruginosa is a common device-associated pathogen and also a model object in biofilm research. In this study biofilm growth dynamics of Pseudomonas aeruginosa RSC-2 strain on different culture media was described. It was shown… read more here.
Keywords: subtilisin like; like proteinase; effect subtilisin; proteinase ... See more keywords
Insight into the binding affinity of thiourea in the calcium binding pocket of proteinase K, through high resolution X-ray crystallography.
Sign Up to like & getrecommendations! Published in 2019 at "Bioorganic chemistry"
DOI: 10.1016/j.bioorg.2019.103443
Abstract: Proteinase K is a stable serine protease, crystallized and extensively used in the study of molecular interactions at the atomic level. During the current study, crystal structure of proteinase K with thiourea (TU) was solved… read more here.
Keywords: calcium binding; thiourea; proteinase; calcium ... See more keywords
Characterization and function analysis of a Kazal-type serine proteinase inhibitor in the red claw crayfish Cherax quadricarinatus.
Sign Up to like & getrecommendations! Published in 2020 at "Developmental and comparative immunology"
DOI: 10.1016/j.dci.2020.103871
Abstract: Kazal-type serine proteinase inhibitors (KPIs) function in physiological and immunological processes requiring proteinase action. In the present study, the first Cherax quadricarinatus KPI gene (designated CqKPI) was identified and characterized. The open reading frame of… read more here.
Keywords: kazal type; type serine; cqkpi; proteinase ... See more keywords
Enhanced inactivation of Salmonella enterica Enteritidis biofilms on the stainless steel surface by proteinase K in the combination with chlorine
Sign Up to like & getrecommendations! Published in 2022 at "Food Control"
DOI: 10.1016/j.foodcont.2021.108519
Abstract: Abstract This study aimed to determine the enzymatic effect of cellulase, DNase I, and proteinase K on Salmonella enterica Enteritidis biofilms on the stainless steel surface at nutrient availability [tryptic soy broth (TSB) and 20… read more here.
Keywords: chlorine; surface; enteritidis biofilms; salmonella enterica ... See more keywords
Technical note: Polyvinylpyrrolidone (PVP) and proteinase-K improve the efficiency of DNA extraction from Japanese larch wood and PCR success rate.
Sign Up to like & getrecommendations! Published in 2021 at "Forensic science international"
DOI: 10.1016/j.forsciint.2021.111005
Abstract: Illegal distribution of timber disrupts the timber market and depletes forest resources. DNA markers are used to verify the legal distribution of wood. However, it is difficult to obtain the quantity and quality of DNA… read more here.
Keywords: proteinase; dna extraction; success rate; dna ... See more keywords
Molecular investigation on the interaction of spermine with proteinase K by multispectroscopic techniques and molecular simulation studies.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2016.10.038
Abstract: The alteration in structure, function and stability of proteinase K in the presence of spermine was investigated using spectroscopic methods and simulation techniques. The stability and enzyme activity of proteinase K-spermine complex were significantly enhanced… read more here.
Keywords: molecular investigation; spermine; proteinase; investigation interaction ... See more keywords
Salt-enhanced processing, proteolytic activity and stability of halophilic thermolysin-like proteinase, salilysin, isolated from a moderate halophile, Chromohalobacter salexigens DSM3043.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.07.050
Abstract: Moderately halophilic bacterium, Chromohalobacter salexigens DSM3043, has a gene Csal_2537 encoding thermolysin-like M4 proteinase. This gene was cloned to pET expression vectors, resulting in high expression of recombinant proteinase, named as salilysin (salinity-dependent thermolysin-like proteinase),… read more here.
Keywords: mature; like proteinase; thermolysin like; proteinase ... See more keywords
Investigation of the Thermal Stability of Proteinase K for the Melt Processing of Poly(l-lactide).
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.2c01008
Abstract: The enzymatic degradation of aliphatic polyesters offers unique opportunities for various use cases in materials science. Although evidently desirable, the implementation of enzymes in technical applications of polyesters is generally challenging due to the thermal… read more here.
Keywords: thermal stability; melt processing; proteinase; stability proteinase ... See more keywords
Structure-Based Screening to Discover New Inhibitors for Papain-like Proteinase of SARS-CoV-2: An In Silico Study
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.0c00836
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) expresses a multifunctional papain-like proteinase (PLpro), which mediates the processing of the viral replicase polyprotein. Inhibition of PLpro has been shown to suppress the viral replication. This study… read more here.
Keywords: like proteinase; cov plpro; study; proteinase ... See more keywords
Inhibitory effect of proteinase K against dermatophyte biofilms: an alternative for increasing the antifungal effects of terbinafine and griseofulvin
Sign Up to like & getrecommendations! Published in 2022 at "Biofouling"
DOI: 10.1080/08927014.2022.2063720
Abstract: Abstract This study aimed to evaluate the effect of proteinase K on mature biofilms of dermatophytes, by assays of metabolic activity and biomass. In addition, the proteinase K–terbinafine and proteinase K–griseofulvin interactions against these biofilms… read more here.
Keywords: dermatophyte biofilms; effect proteinase; terbinafine griseofulvin; proteinase ... See more keywords
Formation and Properties of the Extracellular Proteinase of Aspergillus flavus O-1 Micromycete Active against Fibrillar Proteins
Sign Up to like & getrecommendations! Published in 2021 at "Applied Biochemistry and Microbiology"
DOI: 10.1134/s0003683821050069
Abstract: It has been shown that the Aspergillus flavus O-1 micromycete secretes a complex of at least two proteolytic enzymes. The most active enzyme was isolated via preparative isoelectric focusing and characterized as a serine proteinase… read more here.
Keywords: formation properties; properties extracellular; proteinase; flavus micromycete ... See more keywords