Large-scale analysis of diffusional dynamics of proteins in living yeast cells using fluorescence correlation spectroscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.09.066
Abstract: In the living cells, the majority of proteins does not work alone, but interact with other proteins or other biomolecules to maintain the cellular function, constituting a "protein community". Previous efforts on mass spectroscopy-based protein… read more here.
Keywords: large scale; protein; analysis; proteins living ... See more keywords
Increasing the chemical space of proteins in living cells via genetic code expansion.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2020.07.012
Abstract: In recent years it has become possible to genetically encode an expanded set of designer amino acids with tailored chemical and physical properties (dubbed unnatural amino acids, UAAs) into proteins in living cells by expanding… read more here.
Keywords: increasing chemical; proteins living; chemical space; living cells ... See more keywords
A Toolkit for Engineering Proteins in Living Cells: Peptide with a Tryptophan-Selective Ru-TAP Complex to Regioselectively Photolabel Specific Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c08342
Abstract: Using a chemical approach to crosslink functionally versatile bioeffectors (such as peptides) to native proteins of interest (POI) directly inside a living cell is a useful toolbox for chemical biologists. However, this goal has not… read more here.
Keywords: tap complex; proteins living; living cells; engineering proteins ... See more keywords
Observation of Fluorescent Proteins in Living Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot092767
Abstract: Frequently, there is a need for fluorescent protein detection in mouse cell cultures, including embryonic stem cells or their differentiated derivatives, primary and transformed cells. Here, cells expressing green fluorescent protein-labeled proteins are observed using… read more here.
Keywords: microscopy; fluorescent proteins; proteins living; observation fluorescent ... See more keywords