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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1270-5_11
Abstract: Mitochondria have complex ultrastructure which includes continuous subcompartments, such as matrix, intermembrane space, and two membranes, as well as focal structures, such as nucleoids, RNA granules, and mitoribosomes. Comprehensive studies of the spatial distribution of…
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Keywords:
mitochondrial proteins;
proteins transcripts;
suborganellar localization;
transcripts human ... See more keywords
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Published in 2017 at "Nature Biotechnology"
DOI: 10.1038/nbt.3973
Abstract: We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded…
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Keywords:
quantification proteins;
proteins transcripts;
multiplexed quantification;
transcripts single ... See more keywords