Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2277-3_1
Abstract: Cell signaling governs the basic functions of cells by molecular interactions that involve of many proteins. The abundance of signaling proteins can directly influence cellular responses to external signal, contributing to cellular heterogeneity. Absolute quantification… read more here.
Keywords: using quantitative; absolute quantification; proteins using; signaling proteins ... See more keywords
Ultrasensitive Quantification of Recombinant Proteins Using AAA-MS.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9639-1_1
Abstract: Recombinant proteins are essential components of therapeutic, biotechnological, food, and household products. In some cases, recombinant proteins must be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for… read more here.
Keywords: using aaa; proteins using; quantification; recombinant proteins ... See more keywords
Exploring the folding energy landscapes of heme proteins using a hybrid AWSEM-heme model
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Biological Physics"
DOI: 10.1007/s10867-021-09596-3
Abstract: Heme is an active center in many proteins. Here we explore computationally the role of heme in protein folding and protein structure. We model heme proteins using a hybrid model employing the AWSEM Hamiltonian, a… read more here.
Keywords: heme; heme proteins; proteins using; using hybrid ... See more keywords
An efficient screening method for purifying and crystallizing membrane proteins using modified clear-native PAGE.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.02.007
Abstract: Membrane proteins, such as G-protein coupled receptors, control communication between cells and their environments and are indispensable for many cellular functions. Nevertheless, structural studies on membrane proteins lag behind those on water-soluble proteins, due to… read more here.
Keywords: page; membrane proteins; method; clear native ... See more keywords
Enzymatic hydrolysis of microalgae proteins using serine proteases: A study to characterize kinetic parameters.
Sign Up to like & getrecommendations! Published in 2019 at "Food chemistry"
DOI: 10.1016/j.foodchem.2019.01.111
Abstract: Protein composition and molecular weight play an important role in the digestibility of microalgae proteins. In this study for the first time, proteinous materials of Dunaliella salina and Spirulina platensis were extracted and purified by… read more here.
Keywords: enzymatic hydrolysis; hydrolysis microalgae; microalgae proteins; hydrolysis ... See more keywords
Determination of modification degree of polysialylated therapeutic proteins using 1H-NMR spectroscopy.
Sign Up to like & getrecommendations! Published in 2021 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2021.06.165
Abstract: Water soluble polymers and their derivatives bound to proteins can dramatically favor the biological activity of new drugs and vaccines. Quantification of the modification degree of the protein is crucial during the development and licensing… read more here.
Keywords: degree polysialylated; modification degree; proteins using; spectroscopy ... See more keywords
Sub-Zeptomole Detection of Biomarker Proteins using a Microfluidic Immunoarray with Nanostructured Sensors.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c01507
Abstract: We report here a low-cost electrochemical immunoarray with unprecedented sensitivity in the sub-zeptomole range with up to 5 log-decades dynamic range for accurate, multiplexed protein determinations. The microfluidic array features eight carbon sensors coated with… read more here.
Keywords: biomarker proteins; zeptomole detection; proteins using; sub zeptomole ... See more keywords
Selecting a DNA-Encoded Chemical Library against Non-immobilized Proteins Using a "Ligate-Cross-Link-Purify" Strategy.
Sign Up to like & getrecommendations! Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00343
Abstract: DNA-encoded chemical libraries (DELs) have recently emerged and become an important technology platform in biomedical research and drug discovery. DELs containing large numbers of compounds can be prepared and selected against biological targets to discover… read more here.
Keywords: ligate cross; immobilized proteins; dna encoded; proteins using ... See more keywords
Analyses of Intravesicular Exosomal Proteins Using a Nano-Plasmonic System.
Sign Up to like & getrecommendations! Published in 2018 at "ACS photonics"
DOI: 10.1021/acsphotonics.7b00992
Abstract: Extracellular vesicles (EVs), including exosomes, are nanoscale membrane particles shed from cells and contain cellular proteins whose makeup could inform cancer diagnosis and treatment. Most analyses have focused on surface proteins while analysis of intravesicular… read more here.
Keywords: intravesicular exosomal; using nano; system; proteins using ... See more keywords
Rapid NMR assignments of intrinsically disordered proteins using two-dimensional 13C-detection based experiments.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c9cc03530c
Abstract: An approach for rapid backbone resonance assignments in proteins using only two 2D NMR experiments is presented. The new method involves a combination of high-resolution 13Cα-detected NMR experiments and selective unlabeling of amino acid residues.… read more here.
Keywords: using two; nmr assignments; proteins using; rapid nmr ... See more keywords
Computational identification, characterization and validation of potential antigenic peptide vaccines from hrHPVs E6 proteins using immunoinformatics and computational systems biology approaches
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0196484
Abstract: High-risk human papillomaviruses (hrHPVs) are the most prevalent viruses in human diseases including cervical cancers. Expression of E6 protein has already been reported in cervical cancer cases, excluding normal tissues. Continuous expression of E6 protein… read more here.
Keywords: vaccines hrhpvs; hrhpvs proteins; peptide vaccines; biology ... See more keywords