Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c02215
Abstract: Most proteins in serum are glycosylated, with several annotated as biomarkers and thus diagnostically important and of interest for their role in disease. Most methods for analyzing serum glycoproteins employ either glycan release or glycopeptide… read more here.
Keywords: native mass; mass spectrometry; serum; proteoform ... See more keywords
Improving Proteoform Identifications in Complex Systems Through Integration of Bottom-Up and Top-Down Data.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00332
Abstract: Cellular functions are performed by a vast and diverse set of proteoforms. Proteoforms are the specific forms of proteins produced as a result of genetic variations, RNA splicing, and post-translational modifications (PTMs). Top-down mass spectrometric… read more here.
Keywords: bottom top; analysis; proteoform; proteoform identifications ... See more keywords
1DE-MS Profiling for Proteoform-Correlated Proteomic Analysis, by Combining SDS-PAGE, Whole-Gel Slicing, Quantitative LC-MS/MS, and Reconstruction of Gel Distributions of Several Thousands of Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00180
Abstract: SDS-PAGE has often been used in proteomic analysis, but generally for sample prefractionation although the technique separates proteins by molecular masses (Mws) and the information would contribute to proteoform-level analysis. Here, we report a method… read more here.
Keywords: gel distributions; analysis; gel; sds page ... See more keywords
Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00673
Abstract: Generating top-down tandem mass spectra (MS/MS) from complex mixtures of proteoforms benefits from improvements in fractionation, separation, fragmentation, and mass analysis. The algorithms to match MS/MS to sequences have undergone a parallel evolution, with both… read more here.
Keywords: comparing top; deconvolution; identification; prsm ... See more keywords
New Interface for Faster Proteoform Analysis: Immunoprecipitation Coupled with SampleStream-Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.1c00026
Abstract: Different proteoform products of the same gene can exhibit differing associations with health and disease, and their patterns of modifications may offer more precise markers of phenotypic differences between individuals. However, currently employed protein-biomarker discovery… read more here.
Keywords: proteoform analysis; proteoform; faster proteoform; mass spectrometry ... See more keywords
Highly multiplexed, label-free proteoform imaging of tissues by individual ion mass spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abp9929
Abstract: Imaging of proteoforms in human tissues is hindered by low molecular specificity and limited proteome coverage. Here, we introduce proteoform imaging mass spectrometry (PiMS), which increases the size limit for proteoform detection and identification by… read more here.
Keywords: mass spectrometry; ion; proteoform; proteoform imaging ... See more keywords
Mapping the Proteoform Landscape of Five Human Tissues
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Proteome Research"
DOI: 10.26434/chemrxiv-2022-svlqz
Abstract: A functional understanding of the human body requires structure–function studies of proteins at scale. The chemical structure of proteins is controlled at the transcriptional, translational, and post-translational levels, creating a variety of products with modulated… read more here.
Keywords: proteoform landscape; mapping proteoform; proteoform; landscape five ... See more keywords