Bile proteome analysis by high-precision mass spectrometry to examine novel biomarkers of primary sclerosing cholangitis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of hepato-biliary-pancreatic sciences"
DOI: 10.1002/jhbp.1299
Abstract: BACKGROUND Primary sclerosing cholangitis (PSC) is a chronic inflammatory disease of unknown etiology that affects the intra- and extrahepatic bile ducts. The present study examined the utility of a bile proteome analysis using a high-sensitivity… read more here.
Keywords: bile proteome; psc; proteome analysis; analysis ... See more keywords
Proteome Analysis with Classical 2D-PAGE.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1024-4_5
Abstract: Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is based on the combination of two orthogonal separation techniques. In the first dimension, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF). There are… read more here.
Keywords: dimension; page; classical page; proteome analysis ... See more keywords
Chemical proteomics for subcellular proteome analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.08.001
Abstract: Protein functions are tightly regulated by their subcellular localization and dynamic alteration. Chemical proteomics offers convenience and efficiency for profiling protein features in a native context. In this review, we summarize the recent progress of… read more here.
Keywords: proteomics subcellular; chemical proteomics; proteome analysis; subcellular proteome ... See more keywords
One Sample, One Shot - Evaluation of sample preparation protocols for the mass spectrometric proteome analysis of human bile fluid without extensive fractionation.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2016.11.021
Abstract: The proteome analysis of bile fluid represents a promising strategy to identify biomarker candidates for various diseases of the hepatobiliary system. However, to obtain substantive results in biomarker discovery studies large patient cohorts necessarily need… read more here.
Keywords: sample; bile; fluid; bile fluid ... See more keywords
Shotgun proteome analysis of seminal plasma differentiate boars by reproductive performance.
Sign Up to like & getrecommendations! Published in 2020 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2020.07.013
Abstract: There is a need to identify subfertile boars before they enter into the breeding herd. Seminal plasma proteins are essential for normal sperm function and transport and play an important role in fertilization. The objective… read more here.
Keywords: proteome analysis; total born; farrowing rate; shotgun proteome ... See more keywords
Label-Free Quantification from Direct Infusion Shotgun Proteome Analysis (DISPA-LFQ) with CsoDIAq Software
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c02249
Abstract: Large-scale proteome analysis requires rapid and high-throughput analytical methods. We recently reported a new paradigm in proteome analysis where direct infusion and ion mobility are used instead of liquid chromatography (LC) to achieve rapid and… read more here.
Keywords: dispa lfq; direct infusion; proteome analysis; high throughput ... See more keywords
mNeuCode Empowers Targeted Proteome Analysis of Arginine Dimethylation.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04648
Abstract: Characterization of protein arginine dimethylation presents significant challenges due to its occurrence at the substoichiometric level. To enable a targeted MS/MS analysis of these dimethylation sites, we developed the mNeuCode (methyl-neutron-coding) tag by metabolically labeling… read more here.
Keywords: targeted proteome; dimethylation; arginine dimethylation; proteome analysis ... See more keywords
Increased carvone production in Escherichia coli by balancing limonene conversion enzyme expression via targeted quantification concatamer proteome analysis
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-01469-y
Abstract: (−)-Carvone is a monoterpenoid with a spearmint flavor. A sustainable biotechnological production process for (−)-carvone is desirable. Although all enzymes in (−)-carvone biosynthesis have been functionally expressed in Escherichia coli independently, the yield was low… read more here.
Keywords: production; carvone production; proteome analysis; expression ... See more keywords
Personalised proteome analysis by means of protein microarrays made from individual patient samples
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep39756
Abstract: DNA sequencing has advanced to a state that permits studying the genomes of individual patients as nearly a matter of routine. Towards analysing a tissue’s protein content in a similar manner, we established a method… read more here.
Keywords: protein microarrays; proteome analysis; personalised proteome; analysis means ... See more keywords
Tissue Proteome Analysis of Different Grades of Human Gliomas Provides Major Cues for Glioma Pathogenesis.
Sign Up to like & getrecommendations! Published in 2017 at "Omics A Journal of Integrative Biology"
DOI: 10.1089/omi.2017.0028
Abstract: Abstract Gliomas are heterogeneous and most commonly occurring brain tumors. Blood–brain barrier restricts the entry of brain tumor proteins into blood stream thus limiting the usage of serum or plasma for proteomic analysis. Our study… read more here.
Keywords: tissue proteome; analysis; proteome analysis; analysis different ... See more keywords
Proteome analysis of virulent Aeromonas hydrophila reveals the upregulation of iron acquisition systems in the presence of a xenosiderophore.
Sign Up to like & getrecommendations! Published in 2020 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnaa169
Abstract: The Gram-negative bacterium, Aeromonas hydrophila, has been responsible for extensive losses in the catfish industry for over a decade. Due to this impact, there are ongoing efforts to understand the basic mechanisms that contribute to… read more here.
Keywords: iron acquisition; iron; proteome analysis; dfo ... See more keywords