Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200389
Abstract: Competitive proteome profiling is a powerful approach for the identification of targets of small molecules. This approach usually employs an inhibitor‐derived probe or a cysteine‐reactive probe such as an IA‐alkyne in a comparison between inhibitor‐treated… read more here.
Keywords: comparison different; profiling approaches; competitive proteome; proteome profiling ... See more keywords
Plasma proteome profiling of healthy subjects undergoing bed rest reveals unloading‐dependent changes linked to muscle atrophy
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cachexia, Sarcopenia and Muscle"
DOI: 10.1002/jcsm.13146
Abstract: Inactivity and unloading induce skeletal muscle atrophy, loss of strength and detrimental metabolic effects. Bed rest is a model to study the impact of inactivity on the musculoskeletal system. It not only provides information for… read more here.
Keywords: plasma proteome; muscle; muscle atrophy; bed rest ... See more keywords
Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7698-0_13
Abstract: Proteomics is a technology that allows to decipher the molecular networks involved in the regulation of biological processes such as spermatogenesis. Sertoli cells (SCs) are key players in the paracrine control of this process. Envisioning… read more here.
Keywords: gelc strategy; profiling sertoli; cells using; sertoli cells ... See more keywords
In-tip nanoreactors for cancer cells proteome profiling.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2016.11.021
Abstract: Mass spectrometry (MS)-based proteome profiling is essential for molecular diagnostics in modern biomedical study. To date, sample preparation including protein extraction and proteolysis is still very challenging and lack of efficiency. Recently tips-based sample preparation… read more here.
Keywords: proteolysis; cancer cells; proteome profiling; sample preparation ... See more keywords
Combined morphological and proteome profiling reveals target-independent impairment of cholesterol homeostasis.
Sign Up to like & getrecommendations! Published in 2021 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2021.06.003
Abstract: Unbiased profiling approaches are powerful tools for small-molecule target or mode-of-action deconvolution as they generate a holistic view of the bioactivity space. This is particularly important for non-protein targets that are difficult to identify with… read more here.
Keywords: homeostasis; identification; cholesterol homeostasis; proteome profiling ... See more keywords
Pan-proteome profiling of emerging and re-emerging zoonotic pathogen Orientia tsutsugamushi for getting insight in to microbial pathogenesis.
Sign Up to like & getrecommendations! Published in 2021 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2021.105103
Abstract: With the occurrence and evolution of antibiotic and multidrug resistance in bacteria most of the existing remedies are becoming ineffective. The pan-proteome exploration of the bacterial pathogens helps to identify the wide spectrum therapeutic targets… read more here.
Keywords: pan proteome; orientia tsutsugamushi; pathogen orientia; therapeutic targets ... See more keywords
PACTS-Assisted Thermal Proteome Profiling for Use in Identifying Peptide-Interacting Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00581
Abstract: Bioactive peptides play important roles in various biological processes. However, the traditional methods for profiling the peptide-interacting proteins require modifications to the peptide molecules, often leading to false identifications. We found that the interaction between… read more here.
Keywords: thermal proteome; pacts assisted; interacting proteins; peptide interacting ... See more keywords
Multi-comparative Thermal Proteome Profiling Uncovers New O-GlcNAc Proteins in a System-wide Method.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03371
Abstract: Among diverse protein post-translational modifications, O-GlcNAcylation, a simple but essential monosaccharide modification, plays crucial roles in cellular processes and is closely related to various diseases. Despite its ubiquity in cells, properties of low stoichiometry and… read more here.
Keywords: multi comparative; system wide; comparative thermal; thermal proteome ... See more keywords
Precipitate-Supported Thermal Proteome Profiling Coupled with Deep Learning for Comprehensive Screening of Drug Target Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00936
Abstract: Although thermal proteome profiling (TPP) acts as a popular modification-free approach for drug target deconvolution, some key problems are still limiting screening sensitivity. In the prevailing TPP workflow, only the soluble fractions are analyzed after… read more here.
Keywords: drug target; thermal proteome; drug; precipitate supported ... See more keywords
Thermal Proteome Profiling Reveals the O-GlcNAc-Dependent Meltome.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c10621
Abstract: Posttranslational modifications alter the biophysical properties of proteins and thereby influence cellular physiology. One emerging manner by which such modifications regulate protein functions is through their ability to perturb protein stability. Despite the increasing interest… read more here.
Keywords: thermal proteome; glcnac dependent; proteome profiling; protein ... See more keywords
Complex-centric proteome profiling by SEC-SWATH-MS for the parallel detection of hundreds of protein complexes
Sign Up to like & getrecommendations! Published in 2020 at "Nature Protocols"
DOI: 10.1038/s41596-020-0332-6
Abstract: Most catalytic, structural and regulatory functions of the cell are carried out by functional modules, typically complexes containing or consisting of proteins. The composition and abundance of these complexes and the quantitative distribution of specific… read more here.
Keywords: proteome profiling; complex centric; protein complexes; centric proteome ... See more keywords