Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00682
Abstract: Protein digestion and isotope labeling are two critical steps in proteome quantification. However, the conventional in-solution protocol unavoidably suffers from disadvantages such as time-consuming, low labeling efficiency, and tedious off-line manual operation, which might affect… read more here.
Keywords: graphene oxide; modified polymer; proteome quantification; automated proteome ... See more keywords
Comparative Evaluation of Proteome Discoverer and FragPipe for the TMT-Based Proteome Quantification.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00390
Abstract: Isobaric labeling-based proteomics is widely applied in deep proteome quantification. Among the platforms for isobaric labeled proteomic data analysis, the commercial software Proteome Discoverer (PD) is widely used, incorporating the search engine CHIMERYS, while FragPipe… read more here.
Keywords: proteome quantification; software; quantification; proteome discoverer ... See more keywords
EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification
Sign Up to like & getrecommendations! Published in 2018 at "Nature Methods"
DOI: 10.1038/s41592-018-0037-8
Abstract: We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision… read more here.
Keywords: easi tag; proteome quantification; tag enables; interference free ... See more keywords