Peptide Growth Factor Phytosulfokine-α Stimulates Cell Divisions and Enhances Regeneration from B. oleracea var. capitata L. Protoplast Culture
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Plant Growth Regulation"
DOI: 10.1007/s00344-018-9903-y
Abstract: Protoplasts of six cabbage accessions were isolated from leaf mesophyll and cultured in the presence of 0.01, 0.1 and 1.0 µM phytosulfokine–α (PSK-α) and in a PSK-free control medium. PSK-α was applied for 10 days and later,… read more here.
Keywords: protoplast; medium; regeneration; cell divisions ... See more keywords
A highly efficient stratagem for protoplast isolation and genetic transformation in filamentous fungus Colletotrichum falcatum.
Sign Up to like & getrecommendations! Published in 2022 at "Folia microbiologica"
DOI: 10.1007/s12223-022-00950-z
Abstract: Red rot of sugarcane caused by the hemi-biotrophic fungal pathogen, Colletotrichum falcatum, is a major threat to sugarcane cultivation in many tropical countries such as India, Bangladesh, and Pakistan. With the accumulating information on pathogenicity… read more here.
Keywords: filamentous fungus; colletotrichum falcatum; protoplast; transformation ... See more keywords
An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1
Sign Up to like & getrecommendations! Published in 2017 at "Plant Methods"
DOI: 10.1186/s13007-017-0196-0
Abstract: AbstractBackgroundPerennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient protoplast and transient gene expression… read more here.
Keywords: protoplast; protoplast isolation; protocol; perennial ryegrass ... See more keywords
A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture
Sign Up to like & getrecommendations! Published in 2022 at "Micromachines"
DOI: 10.3390/mi13122109
Abstract: Protoplasts are widely used in gene function verification, subcellular localization, and single-cell sequencing because of their complete physiological activities. The traditional methods based on tissues and organs cannot satisfy the requirement. Therefore, the isolation and… read more here.
Keywords: microfluidic chip; protoplast; dual channel; chip ... See more keywords
Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation
Sign Up to like & getrecommendations! Published in 2021 at "Plants"
DOI: 10.3390/plants10020375
Abstract: Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal… read more here.
Keywords: shoot root; protoplast; plant; cell cycle ... See more keywords