An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1
Sign Up to like & getrecommendations! Published in 2017 at "Plant Methods"
DOI: 10.1186/s13007-017-0196-0
Abstract: AbstractBackgroundPerennial ryegrass (Lolium perenne L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient protoplast and transient gene expression… read more here.
Keywords: protoplast; protoplast isolation; protocol; perennial ryegrass ... See more keywords
Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays
Sign Up to like & getrecommendations! Published in 2020 at "Plant Methods"
DOI: 10.1186/s13007-020-00692-4
Abstract: Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings… read more here.
Keywords: transfection; pause points; protoplast isolation; gene expression ... See more keywords
Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Sign Up to like & getrecommendations! Published in 2021 at "Agriculture"
DOI: 10.3390/agriculture11080699
Abstract: Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and biotechnology. In… read more here.
Keywords: plant; protoplast isolation; fusion; culture ... See more keywords
Optimized and Reliable Protoplast Isolation for Transient Gene Expression Studies in the Gymnosperm Tree Species Pinus densiflora
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DOI: 10.3390/f16091373
Abstract: Efficient protoplast isolation and gene transfection remain significant challenges in gymnosperms, particularly in Pinus species, where stable transformation is highly limited. Conventional pine protoplast preparation methods have resulted in extremely low transfection efficiencies, hindering functional… read more here.
Keywords: gene expression; gene; transient gene; protoplast isolation ... See more keywords
An Efficient System for Mesophyll Protoplast Isolation, Purification, and Transformation in Loquat: Studies on Fluorescent Marker Analysis and Subcellular Localization
Sign Up to like & getrecommendations! Published in 2025 at "Horticulturae"
DOI: 10.3390/horticulturae11040391
Abstract: Loquat (Eriobotrya japonica Lindl.) is one of the most important subtropical evergreen fruit trees. However, due to the lack of widely applicable genetic transformation platforms, the research about gene functional characterization and molecular mechanisms is… read more here.
Keywords: system; efficient system; protoplast isolation; loquat ... See more keywords
Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23020837
Abstract: The cultivated peanut (Arachis hypogaea L.) is a legume consumed worldwide in the form of oil, nuts, peanut butter, and candy. Improving peanut production and nutrition will require new technologies to enable novel trait development.… read more here.
Keywords: genome editing; isolation transformation; protoplast isolation; allergen gene ... See more keywords