Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a.
Sign Up to like & getrecommendations! Published in 2022 at "Nature biomedical engineering"
DOI: 10.1038/s41551-022-00861-x
Abstract: CRISPR-based assays for the detection of nucleic acids are highly specific, yet they are not fast, sensitive or easy to use. Here we report a one-step fluorescence assay for the detection of severe acute respiratory… read more here.
Keywords: cov rna; sars cov; fast sensitive; detection ... See more keywords
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
Sign Up to like & getrecommendations! Published in 2020 at "Science"
DOI: 10.1126/science.aba8853
Abstract: A PAMless base editor CRISPR-Cas DNA base editing typically requires a specific motif for targeting known as a protospacer-adjacent motif (PAM). This requirement limits the sequences within a genome that can be targeted. Walton et… read more here.
Keywords: near pamless; adjacent motif; crispr; protospacer adjacent ... See more keywords