BioID: A Proximity-Dependent Labeling Approach in Proteomics Study.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8814-3_10
Abstract: Biological activities are mainly executed by proteins and in most of the occasions these activities are accomplished by protein complexes or through protein-protein interactions (PPI). So it is critical to reveal how the protein complexes… read more here.
Keywords: protein; target protein; interacting partners; dependent labeling ... See more keywords
Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.10.017
Abstract: The use of proximity-dependent biotinylation approaches combined with mass spectrometry (e.g. BioID and APEX) has revolutionized the study of protein-protein interactions and organellar proteomics. These powerful techniques are based on the fusion of an enzyme… read more here.
Keywords: protein; dependent biotinylation; neighborhood; proximity dependent ... See more keywords
Exploring Options for Proximity-Dependent Biotinylation Experiments: Comparative Analysis of Labeling Enzymes and Affinity Purification Resins
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.3c00908
Abstract: Proximity-dependent biotinylation (PDB) techniques provide information about the molecular neighborhood of a protein of interest, yielding insights into its function and localization. Here, we assessed how different labeling enzymes and streptavidin resins influence PDB results.… read more here.
Keywords: dependent biotinylation; proximity dependent; resin; labeling enzymes ... See more keywords
A Kinetic View on Proximity-Dependent Selectivity of Carbon Dioxide Reduction on Bifunctional Catalysts
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DOI: 10.1021/acscatal.0c03414
Abstract: Multifunctional catalysts with distinct functional components are known to have much improved selectivity. However, the well-known proximity-dependent selectivity observed in several high-profile e... read more here.
Keywords: selectivity; kinetic view; dependent selectivity; view proximity ... See more keywords
Proximity-dependent biotin labelling reveals CP190 as an EcR/Usp molecular partner
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-61514-0
Abstract: Proximity-dependent biotin labelling revealed undescribed participants of the ecdysone response in Drosophila . Two labelling enzymes (BioID2 and APEX2) were fused to EcR or Usp to biotin label the surrounding proteins. The EcR/Usp heterodimer was… read more here.
Keywords: biotin labelling; ecr usp; dependent biotin; proximity dependent ... See more keywords
An APEX2-based proximity-dependent biotinylation assay with temporal specificity to study protein interactions during autophagy in the yeast Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2024 at "Autophagy"
DOI: 10.1080/15548627.2024.2366749
Abstract: ABSTRACT Autophagosome biogenesis is a complex process orchestrated by dynamic interactions between Atg (autophagy-related) proteins and characterized by the turnover of specific cargoes, which can differ over time and depending on how autophagy is stimulated.… read more here.
Keywords: protein; temporal specificity; dependent biotinylation; proximity ... See more keywords
Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.852911
Abstract: In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms. The associations between specific… read more here.
Keywords: biotinylation approaches; proximity dependent; proximity; approaches explore ... See more keywords
Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular Neuroscience"
DOI: 10.3389/fncel.2022.801644
Abstract: Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given… read more here.
Keywords: biotinylation; proximity dependent; dependent biotinylation; biotinylation living ... See more keywords