Fast and Dynamic Mapping of the Protein Corona on Nanoparticle Surfaces by Photocatalytic Proximity Labeling
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202206636
Abstract: Protein corona broadly affects the delivery of nanomedicines in vivo. Although it has been widely studied by multiple strategies like centrifugal sedimentation, the rapidly forming mechanism and the dynamic structure of the protein corona at… read more here.
Keywords: nano ppl; fast dynamic; photocatalytic proximity; proximity labeling ... See more keywords
Photocatalytic Proximity Labeling for Profiling the Subcellular Organization of Biomolecules
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202200745
Abstract: Investigating the subcellular organization of biomolecules is important for understanding their biological functions. Over the past decade, proximity‐dependent labeling methods have emerged as powerful tools for mapping biomolecules in their native context. These methods often… read more here.
Keywords: subcellular organization; proximity; organization biomolecules; photocatalytic proximity ... See more keywords
The EMARS Reaction for Proximity Labeling.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9537-0_1
Abstract: To understand cellular processes at molecular levels, elucidation of protein-protein interactions occurring at a specific location in living cells is required. We have developed a proximity labeling method mediated by the enzyme-mediated activation of radical… read more here.
Keywords: emars reaction; proximity labeling; reaction proximity; biology ... See more keywords
Identification of Lipid Droplet Proteomes by Proximity Labeling Proteomics Using APEX2.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9537-0_5
Abstract: Lipid droplets (LDs) are ubiquitous lipid storage organelles composed of a neutral lipid core surrounded by a phospholipid monolayer that is decorated with integral and peripheral proteins. Accurate identification of LD proteins using biochemical fractionation… read more here.
Keywords: proximity labeling; lipid droplet; identification lipid; droplet proteomes ... See more keywords
Identification by proximity labeling of novel lipidic and proteinaceous potential partners of the dopamine transporter
Sign Up to like & getrecommendations! Published in 2021 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-021-03998-1
Abstract: Dopamine (DA) transporters (DATs) are regulated by trafficking and modulatory processes that probably rely on stable and transient interactions with neighboring proteins and lipids. Using proximity-dependent biotin identification (BioID), we found novel potential partners for… read more here.
Keywords: labeling novel; proximity labeling; identification proximity; proximity ... See more keywords
Proteomic navigation using proximity-labeling.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.03.028
Abstract: The identification of bona fide protein-protein interactions and the mapping of proteomes was greatly enhanced by protein tagging for generic affinity purification methods and analysis by mass spectrometry (AP-MS). The high quality of AP-MS data… read more here.
Keywords: protein protein; proximity labeling; proteomic navigation; proximity ... See more keywords
Molecular Spatiomics by Proximity Labeling
Sign Up to like & getrecommendations! Published in 2022 at "Accounts of Chemical Research"
DOI: 10.1021/acs.accounts.2c00061
Abstract: Conspectus Proximity labeling can be defined as an enzymatic “in-cell” chemical reaction that catalyzes the proximity-dependent modification of biomolecules in live cells. Since the modified proteins can be isolated and identified via mass spectrometry, this… read more here.
Keywords: cell; reaction; proximity; chemistry ... See more keywords
Selective and Nongenetic Peroxidase Tag of Membrane Protein: a Nucleic Acid Tool for Proximity Labeling.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04148
Abstract: The protein nanoenvironment on the plasma membrane is intimately linked to cellular biological functions. Elucidation of the protein nanoenvironment contributes to understanding the pathological mechanism and discovery of disease biomarkers. However, methods enabling characterization of… read more here.
Keywords: tool; proximity labeling; nucleic acid; protein ... See more keywords
Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01042
Abstract: Deciphering the endogenous interactors of histone post-translational modifications (hPTMs, also called histone marks) is essential to understand the mechanisms of epigenetic regulation. However, most of the analytical methods to determine hPTM interactomes are in vitro… read more here.
Keywords: histone marks; histone; genetic code; code expansion ... See more keywords
Shared and Distinctive Neighborhoods of Emerin and Lamin B Receptor Revealed by Proximity Labeling and Quantitative Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.2c00281
Abstract: Emerin and lamin B receptor (LBR) are abundant transmembrane proteins of the nuclear envelope that are concentrated at the inner nuclear membrane (INM). Although both proteins interact with chromatin and nuclear lamins, they have distinctive… read more here.
Keywords: proximity; lamin receptor; neighborhoods emerin; quantitative proteomics ... See more keywords
μMap-Red: Proximity Labeling by Red Light Photocatalysis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c01384
Abstract: Modern proximity labeling techniques have enabled significant advances in understanding biomolecular interactions. However, current tools primarily utilize activation modes that are incompatible with complex biological environments, limiting our ability to interrogate cell- and tissue-level microenvironments… read more here.
Keywords: proximity labeling; red light; red proximity; map red ... See more keywords