How chromosome topologies get their shape: views from proximity ligation and microscopy methods
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Letters"
DOI: 10.1002/1873-3468.13961
Abstract: The 3D organization of our genome is an important determinant for the transcriptional output of a gene in (patho)physiological contexts. The spatial organization of linear chromosomes within nucleus is dominantly inferred using two distinct approaches,… read more here.
Keywords: proximity ligation; microscopy; ligation; dna fish ... See more keywords
Proximity Ligation Assay for Detecting Protein‐Protein Interactions and Protein Modifications in Cells and Tissues in Situ
Sign Up to like & getrecommendations! Published in 2020 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.115
Abstract: Biochemical methods can reveal stable protein‐protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein‐protein interactions in cells and tissues in situ provides important additional… read more here.
Keywords: protein; proximity ligation; protein interactions; ligation assay ... See more keywords
Metagenomes Binning Using Proximity-Ligation Data.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1390-0_8
Abstract: Microbial communities are key components of all ecosystems, but characterization of their complete genomic structure remains challenging. Typical analysis tends to elude the complexity of the mixes in terms of species, strains, as well as… read more here.
Keywords: using proximity; binning using; metagenomes binning; proximity ligation ... See more keywords
Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6780-3_5
Abstract: Distribution of the death receptor CD95 into lipid rafts (aggregation) and/or its internalization may contribute to the implementation of the apoptotic signal at the detriment of the non-apoptotic signaling pathway [1-6]. Also CD95 can form… read more here.
Keywords: assay pla; pla; proximity ligation; ligation assay ... See more keywords
Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-020-11049-1
Abstract: It is of great significance to establish sensitive and accurate pathogen detection methods, considering the continuous emergence or re-emergence of infectious diseases seriously influences the safety of human and animals. Proximity ligation assay (PLA) is… read more here.
Keywords: protein; detection; proximity ligation; ligation assay ... See more keywords
Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.04.009
Abstract: Antibody-based in situ proximity ligation assays (isPLA) have the potential to study protein phosphorylation and protein interactions with spatial resolution in intact tissues. However, the application of isPLA at the tissue level is limited by… read more here.
Keywords: proximity ligation; smooth muscle; situ proximity; protein interactions ... See more keywords
Proximity ligation assay induced hairpin to DNAzyme structure switching for entropy-driven amplified detection of thrombin.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.03.007
Abstract: A proximity ligation assay (PLA) induced hairpin to DNAzyme structure switching strategy has been described for entropy-driven amplified detection of thrombin. The enzyme-strand (E-DNA) and substrate-strand (S-DNA) of DNAzyme are locked in hairpins structure, and… read more here.
Keywords: dnazyme; proximity ligation; entropy driven; structure ... See more keywords
Proximity ligation assays for precise quantification of femtomolar proteins in single cells using self-priming microfluidic dPCR chip.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.05.034
Abstract: The quantification of low concentration proteins can facilitate the discovery of some significant biomarkers, and provide us a more profound understanding of cell heterogeneity when applied to single cell analysis. However, most state-of- art single… read more here.
Keywords: proximity ligation; microfluidic dpcr; cell; quantification ... See more keywords
Multi-contact 4C: long-molecule sequencing of complex proximity ligation products to uncover local cooperative and competitive chromatin topologies
Sign Up to like & getrecommendations! Published in 2020 at "Nature Protocols"
DOI: 10.1038/s41596-019-0242-7
Abstract: We present the experimental protocol and data analysis toolbox for multi-contact 4C (MC-4C), a new proximity ligation method tailored to study the higher-order chromatin contact patterns of selected genomic sites. Conventional chromatin conformation capture (3C)… read more here.
Keywords: proximity ligation; chromatin; analysis; ligation products ... See more keywords
Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-01516-7
Abstract: Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine. Capillary isoelectric focusing together with antibody-based detection can resolve and identify proteins… read more here.
Keywords: protein; detection; proximity ligation; capillary isoelectric ... See more keywords
Proximity ligation-transcription circuit-powered exponential amplifications for single-molecule monitoring of telomerase in human cells.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical communications"
DOI: 10.1039/d2cc06087f
Abstract: We develop a new strategy for single-molecule monitoring of telomerase based on proximity ligation-transcription circuit-powered exponential amplifications. This strategy exhibits high sensitivity with a detection limit of 0.1 aM for the synthetic telomerase product TPC4… read more here.
Keywords: single molecule; molecule monitoring; monitoring telomerase; ligation transcription ... See more keywords