EDTA is superior to DTT treatment for overcoming the prozone effect in HLA antibody testing
Sign Up to like & getrecommendations! Published in 2017 at "HLA"
DOI: 10.1111/tan.12950
Abstract: A limitation of solid‐phase human leukocyte antigen (HLA) antibody assays is the falsely low/negative result of samples with high‐titer antibodies, a phenomenon known as the prozone effect. Here we compared the efficacy of ethylenediaminetetraacetic acid… read more here.
Keywords: dtt treatment; prozone effect; prozone; hla ... See more keywords
EDTA Treatment for Overcoming the Prozone Effect and for Predicting C1q Binding in HLA Antibody Testing
Sign Up to like & getrecommendations! Published in 2019 at "Annals of Laboratory Medicine"
DOI: 10.3343/alm.2019.39.6.572
Abstract: The Luminex-based single antigen bead (SAB) assay is widely used to detect HLA antibody in transplant recipients. However, one limitation of the SAB assay is the prozone effect, which occurs mostly as a result of… read more here.
Keywords: effect; prozone effect; antibody; edta treatment ... See more keywords