A sensitive and radiolabeling-free method for pseudouridine detection.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.113350
Abstract: Existing methodologies for detecting Pseudouridine (Ψ) mostly use CMCT labeling or radiolabeling. Described herein is a sensitive and quantitative method for Ψ detection that does not need this labelling. This approach combines the selectivity of… read more here.
Keywords: detection; sensitive radiolabeling; pseudouridine; method ... See more keywords
Decoding pseudouridine: an emerging target for therapeutic development.
Sign Up to like & getrecommendations! Published in 2022 at "Trends in pharmacological sciences"
DOI: 10.1016/j.tips.2022.03.008
Abstract: Pseudouridine (Ψ) is the most abundant post-transcriptional RNA modification and is widespread in multiple RNA species. Ψ impacts various aspects of RNA biology, conferring distinct structural and functional properties to the RNA molecules that it… read more here.
Keywords: pseudouridine; emerging target; therapeutic development; decoding pseudouridine ... See more keywords
Computational and NMR studies of RNA duplexes with an internal pseudouridine-adenosine base pair
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-52637-0
Abstract: Pseudouridine (Ψ) is the most common chemical modification present in RNA. In general, Ψ increases the thermodynamic stability of RNA. However, the degree of stabilization depends on the sequence and structural context. To explain experimentally… read more here.
Keywords: pseudouridine; rna duplexes; base pair; duplexes internal ... See more keywords
Interferon inducible pseudouridine modification in human mRNA by quantitative nanopore profiling
Sign Up to like & getrecommendations! Published in 2021 at "Genome Biology"
DOI: 10.1186/s13059-021-02557-y
Abstract: Pseudouridine (Ψ) is an abundant mRNA modification in mammalian transcriptome, but its functions have remained elusive due to the difficulty of transcriptome-wide mapping. We develop a nanopore native RNA sequencing method for quantitative Ψ prediction… read more here.
Keywords: pseudouridine modification; interferon inducible; inducible pseudouridine; pseudouridine ... See more keywords
A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability
Sign Up to like & getrecommendations! Published in 2017 at "EMBO reports"
DOI: 10.15252/embr.201643391
Abstract: Pseudouridylation is a common post‐transcriptional modification in RNA, but its functional consequences at the cellular level remain largely unknown. Using a proximity‐biotinylation assay, we identified a protein module in mitochondrial RNA granules, platforms for post‐transcriptional… read more here.
Keywords: protein; mitochondrial protein; pseudouridine; protein synthesis ... See more keywords
Structure Characterization of Escherichia coli Pseudouridine Kinase PsuK
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.926099
Abstract: Pseudouridine (Ψ) is one of the most abundant RNA modifications in cellular RNAs that post-transcriptionally impact many aspects of RNA. However, the metabolic fate of modified RNA nucleotides has long been a question. A pseudouridine… read more here.
Keywords: structure; pseudouridine kinase; kinase psuk; escherichia coli ... See more keywords
A Pseudouridine Isoxazolidinyl Nucleoside Analogue Structural Analysis: A Morphological Approach
Sign Up to like & getrecommendations! Published in 2018 at "Molecules"
DOI: 10.3390/molecules23123381
Abstract: An in silico study has been conducted upon (3′RS,5′SR)-5-[2′-benzyl-5′-hydroxymethyl-1′,2′-isoxazolidin-3′-yl]uracil through a molecular dynamics/docking approach that highlights its potential inhibitory activity upon the wild-type pseudouridine 5′-monophosphate glycosidase. The crystal structure of this compound has been solved… read more here.
Keywords: pseudouridine; pseudouridine isoxazolidinyl; isoxazolidinyl nucleoside; approach ... See more keywords