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PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries

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1 Published in 2019 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms20163908

Abstract: QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. This efficient and cost-effective method… read more here.

Keywords: mutagenesis library; quickstep; random mutagenesis; pto quickstep ... See more keywords