Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "Electronic Journal of Biotechnology"
DOI: 10.1016/j.ejbt.2017.07.001
Abstract: Abstract Background Pullulanase production in both wild-type strains and recombinantly engineered strains remains low. The Shine-Dalgarno (SD) sequence and stem-loop structure in the 5′ or 3′ untranslated region (UTR) are well-known determinants of mRNA stability.… read more here.
Keywords: pullulanase; mrna stability; pula pula; expression ... See more keywords
A pH-stable, detergent and chelator resistant type I pullulanase from Bacillus pseudofirmus 703 with high catalytic efficiency.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.11.139
Abstract: Pullulanase with high catalytic efficiency has attracted great attention from both the academic and industrial communities for its wide application in cold starch hydrolysis. A novel pullulanase gene pul703 was cloned from a mesophilic bacteria… read more here.
Keywords: pullulanase; bacillus pseudofirmus; high catalytic; pul703 ... See more keywords
In vitro digestibility and quality attributes of white salted noodles supplemented with pullulanase-treated flour.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.11.043
Abstract: This study aimed to develop a noodle with slow digestibility by adding pullulanase-treated wheat flour (PF). PF was prepared from normal wheat flour (NF) by pullulanase treatment, then its characteristics were compared with NF. Varying… read more here.
Keywords: pullulanase; vitro digestibility; white salted; pullulanase treated ... See more keywords
Functional characterization of tryptophan437 at subsite +2 in pullulanase from Bacillus subtilis str. 168.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.04.103
Abstract: Pullulanase, a typical debranching enzyme, specifically hydrolyzes α-1,6 glycosidic linkages in pullulan, starch and so on. There is accumulated knowledge about the catalysis of pullulanase, but functional roles of acceptor subsites get less attention. According… read more here.
Keywords: pullulanase; pullulanase bacillus; subtilis str; bacillus subtilis ... See more keywords
Safety evaluation of the food enzyme pullulanase from a genetically modified Bacillus licheniformis (strain DP‐Dzp39)
Sign Up to like & getrecommendations! Published in 2019 at "EFSA Journal"
DOI: 10.2903/j.efsa.2019.5554
Abstract: Abstract The food enzyme pullulanase (pullulan 6‐α‐glucanohydrolase; EC 3.2.1.41) is produced with a genetically modified Bacillus licheniformis (strain DP‐Dzp39) by Danisco US Inc. The genetic modifications do not give rise to safety concerns. The food… read more here.
Keywords: pullulanase; safety; exposure; food ... See more keywords
Cassava pullulanase and its synergistic debranching action with isoamylase 3 in starch catabolism
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2023.1114215
Abstract: Pullulanase (EC 3.2.1.41, PUL), a debranching enzyme belonging to glycoside hydrolase family 13 subfamily 13, catalyses the cleavage of α-1,6 linkages of pullulan and β-limit dextrin. The present work studied PUL from cassava Manihot esculenta… read more here.
Keywords: isoamylase; limit dextrin; starch; starch catabolism ... See more keywords