Monitoring of the Cytoskeleton-Dependent Intracellular Trafficking of Fluorescent Iron Oxide Nanoparticles by Nanoparticle Pulse-Chase Experiments in C6 Glioma Cells
Sign Up to like & getrecommendations! Published in 2018 at "Neurochemical Research"
DOI: 10.1007/s11064-018-2627-3
Abstract: Iron oxide nanoparticles (IONPs) are used for various biomedical and therapeutic approaches. To investigate the uptake and the intracellular trafficking of IONPs in neural cells we have performed nanoparticle pulse-chase experiments to visualize the internalization… read more here.
Keywords: nanoparticle pulse; fluorescent; pulse chase; iron ... See more keywords
Time-Resolved Tracking of Separately Internalized Neuropeptide Y2 Receptors by Two-Color Pulse-Chase.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00999
Abstract: Internalization and intracellular trafficking of G protein-coupled receptors (GPCR) plays an important role in the signal transduction. These processes are often highly dynamic and take place rapidly. In the past 10 years, it became obvious… read more here.
Keywords: two color; time; pulse chase; color pulse ... See more keywords
Pulse-Chase Labeling of Protein Antigens with [35S]Methionine.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot098525
Abstract: Pulse-chase labeling of antigens with [35S]methionine is used to determine the relative half-life of a protein. In this protocol, intracellular unlabeled methionine levels are reduced by starvation of cells for 0.5-1 h, and then the… read more here.
Keywords: methionine; pulse chase; 35s methionine; chase labeling ... See more keywords