Coupling Nanostructured Microchips with Covalent Chemistry Enables Purification of Sarcoma-Derived Extracellular Vesicles for Downstream Functional Studies.
Sign Up to like & getrecommendations! Published in 2020 at "Advanced functional materials"
DOI: 10.1002/adfm.202003237
Abstract: Tumor-derived extracellular vesicles (EVs) play essential roles in intercellular communication during tumor growth and metastatic evolution. Currently, little is known about the possible roles of tumor-derived EVs in sarcoma because the lack of specific surface… read more here.
Keywords: sarcoma; extracellular vesicles; chemistry; derived extracellular ... See more keywords
An Ideal Molecular Sieve for Acetylene Removal from Ethylene with Record Selectivity and Productivity.
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DOI: 10.1002/adma.201704210
Abstract: Realization of ideal molecular sieves, in which the larger gas molecules are completely blocked without sacrificing high adsorption capacities of the preferred smaller gas molecules, can significantly reduce energy costs for gas separation and purification… read more here.
Keywords: ethylene; ideal; record; acetylene ... See more keywords
Exosomes for Wound Healing: Purification Optimization and Identification of Bioactive Components
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DOI: 10.1002/advs.202002596
Abstract: Abstract Human mesenchymal stem cell exosomes have been shown to promote cutaneous wound healing. Their bioactivity is most often attributed to their protein and nucleic acid components, while the function of exosomal lipids remains comparatively… read more here.
Keywords: wound healing; exosomes wound; optimization; purification ... See more keywords
A novel system for separation and purification of egg pigments from the nondiapause red-egg mutant Re-nd in the silkworm, Bombyx mori.
Sign Up to like & getrecommendations! Published in 2020 at "Archives of insect biochemistry and physiology"
DOI: 10.1002/arch.21728
Abstract: Re-nd, which was induced from the wild-type C108 by the chemical mutagen N-methane-N-methylnitrourea, is a nondiapause red-egg mutant of silkworm Bombyx mori. The special significance of the Re-nd mutant is that it is an independent… read more here.
Keywords: silkworm; nondiapause red; egg mutant; egg ... See more keywords
Purification of cell-derived japanese encephalitis virus by dual-mode chromatography.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.1960
Abstract: Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scaleup. Here, a rapid, simple and… read more here.
Keywords: chromatography; japanese encephalitis; purification; encephalitis virus ... See more keywords
Approaches to purification and concentration of rAAV vectors for gene therapy
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DOI: 10.1002/bies.202200019
Abstract: Recombinant adeno‐associated viruses (rAAVs) are promising vectors for the delivery of various genetic constructs into eukaryotic cells. rAAVs have a number of properties that make it possible to successfully use them both in vitro and… read more here.
Keywords: purification; raav vectors; concentration raav; purification concentration ... See more keywords
Characterisation of the E. coli HMS174 and BLR host cell proteome to guide purification process development.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202300068
Abstract: Mass-spectrometry-based proteomics is increasingly employed to monitor purification processes or to detect critical host cell proteins in the final drug substance. This approach is inherently unbiased and can be used to identify individual host cell… read more here.
Keywords: purification; host cell; process development;
Single‐step purification of cyclotides using affinity chromatography
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DOI: 10.1002/bip.23010
Abstract: Cyclotides are considered promising scaffolds for drug development owing to their inherent host defence activities and highly stable structure, defined by the cyclic cystine knot. These proteins are expressed as complex mixtures in plants. Although… read more here.
Keywords: purification cyclotides; purification; affinity chromatography; cyclotides using ... See more keywords
The future of protein scaffolds as affinity reagents for purification
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DOI: 10.1002/bit.26090
Abstract: Affinity purification is one of the most powerful separation techniques extensively employed both at laboratory and production scales. While antibodies still represent the gold standard affinity reagents, others derived from non‐immunoglobulin scaffolds emerged as interesting… read more here.
Keywords: immunoglobulin scaffolds; affinity; affinity purification; affinity reagents ... See more keywords
Comparison of purification strategies for antibodies used in a broad spectrum host cell protein immunoassay
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DOI: 10.1002/bit.26482
Abstract: Host cell proteins (HCPs) are a heterogeneous mixture of impurities that should be minimized in bulk preparations of biotechnologically produced medicines. Immunoassays are commonly used to detect and measure HCPs in therapeutic products, and a… read more here.
Keywords: host cell; purification; comparison purification; purification strategies ... See more keywords
An impurity characterization based approach for the rapid development of integrated downstream purification processes
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DOI: 10.1002/bit.26718
Abstract: In this study, we describe a new approach for the characterization of process‐related impurities along with an in silico tool to generate orthogonal, integrated downstream purification processes for biological products. A one‐time characterization of process‐related… read more here.
Keywords: integrated downstream; impurity; approach; purification ... See more keywords