Purification of Antibodies Using Affinity Chromatography.
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DOI: 10.1007/978-1-4939-6412-3_15
Abstract: Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilized to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It… read more here.
Keywords: protein; purification antibodies; affinity chromatography; affinity ... See more keywords
Purification of Antibodies: Diethylaminoethyl (DEAE) Chromatography.
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DOI: 10.1101/pdb.prot099135
Abstract: Because IgG from most species (other than rodents) tends to have an isoelectric point around neutral, two approaches can be used when separating IgG using diethylaminoethyl (DEAE) resins. When serum containing antibodies is applied to… read more here.
Keywords: purification antibodies; diethylaminoethyl deae; column; igg ... See more keywords
Protein A and Protein G Purification of Antibodies.
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DOI: 10.1101/pdb.prot099143
Abstract: Protein A and Protein G are immunoglobulin-binding proteins expressed in Staphylococcus aureus and Streptococcus sp., respectively, that have been adapted for use in purifying large amounts of IgG. They are available covalently attached to affinity… read more here.
Keywords: protein; purification antibodies; affinity; protein protein ... See more keywords