Articles with "purification biochemical" as a keyword



Purification and biochemical characterization of an extracellular β-d-fructofuranosidase from Aspergillus sp.

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Published in 2018 at "3 Biotech"

DOI: 10.1007/s13205-018-1109-2

Abstract: This study focused on the purification and characterization of an extracellular β-d-fructofuranosidase or invertase from Aspergillus sojae JU12. The protein was purified by size exclusion chromatography with 5.41 fold and 10.87% recovery. The apparent molecular… read more here.

Keywords: characterization extracellular; fructofuranosidase; extracellular fructofuranosidase; purification biochemical ... See more keywords
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Purification and biochemical characteristics of the microbial extracellular protease from Lactobacillus curvatus isolated from Harbin dry sausages.

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Published in 2019 at "International journal of biological macromolecules"

DOI: 10.1016/j.ijbiomac.2019.04.169

Abstract: This study investigates the purification and biochemical characteristics of the protease secreted by Lactobacillus curvatus R5, which was isolated from Harbin dry sausages. The optimized fermentation conditions were fermentation time 36 h, initial pH 6 and fermentation… read more here.

Keywords: protease; biochemical characteristics; dry sausages; harbin dry ... See more keywords
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Production, purification and biochemical characterization of the microbial protease produced by Lactobacillus fermentum R6 isolated from Harbin dry sausages

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Published in 2020 at "Process Biochemistry"

DOI: 10.1016/j.procbio.2019.10.029

Abstract: Abstract This study investigated the purification and biochemical characterization of the protease produced by Lactobacillus fermentum R6 isolated from Harbin dry sausages. The optimized fermentation conditions were as follows: a fermentation time of 48 h, an… read more here.

Keywords: protease produced; protease; dry sausages; harbin dry ... See more keywords
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Soluble expression, purification and biochemical characterization of a C-7 cholesterol dehydrogenase from Drosophila melanogaster

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Published in 2019 at "Steroids"

DOI: 10.1016/j.steroids.2019.108495

Abstract: The C-7 cholesterol dehydrogenase (NVD), which converts cholesterol to 7-dehydrocholesterol (7-DHC) by 7,8-dehydrogenation, plays a pivotal role in the metabolism of cholesterol and steroid intermediates. The NVD protein was successfully expressed in insect Sf9 cells.… read more here.

Keywords: cholesterol; cholesterol dehydrogenase; soluble expression; expression purification ... See more keywords
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Extraction, purification, and biochemical characterization of serine protease from leaves of Abrus precatorius

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Published in 2017 at "Preparative Biochemistry and Biotechnology"

DOI: 10.1080/10826068.2017.1373289

Abstract: ABSTRACT A protease from fresh leaves of Abrus precatorius was purified using two classical chromatography techniques: ion-exchange (DEAE-Sepharose) and Gel filtration (Sephadex G-75). The purified protease showed a molecular weight of ∼ 28 kDa on sodium dodecyl… read more here.

Keywords: extraction purification; protease; purified protease; leaves abrus ... See more keywords
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Purification and biochemical analysis of native AMPA receptors from three different mammalian species

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Published in 2022 at "PLOS ONE"

DOI: 10.1101/2022.09.16.508249

Abstract: The majority of fast, excitatory synaptic transmission in the central nervous system (CNS) is mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), which are glutamate-activated ion channels integral to synaptic plasticity, motor coordination, learning, and memory. AMPARs… read more here.

Keywords: analysis native; purification biochemical; native ampa; three different ... See more keywords

Purification and Biochemical Characterization of a New Protease Inhibitor from Conyza dioscoridis with Antimicrobial, Antifungal and Cytotoxic Effects

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Published in 2020 at "Molecules"

DOI: 10.3390/molecules25225452

Abstract: The main objective of the current study was the extraction, purification, and biochemical characterization of a protein protease inhibitor from Conyza dioscoridis. Antimicrobial potential and cytotoxic effects were also examined. The protease inhibitor was extracted… read more here.

Keywords: protease inhibitor; inhibitor; pdinhibitor; biochemical characterization ... See more keywords