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Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2017.08.014
Abstract: Functional studies of extracellular proteins are often performed using coimmunoprecipitation without purified proteins. However, in order to exclude unspecific reactions of contaminants and for quantitative analysis of specific functions, it is necessary to use purified…
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Keywords:
purification recombinant;
recombinant fibulins;
expression;
mammalian cells ... See more keywords
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Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2017.08.020
Abstract: The matricellular protein SPARC (secreted protein acidic and rich in cysteine, also known as osteonectin or as BM-40) is a collagen-binding protein with a capacity to induce cell rounding and influence proliferation in cultured cells.…
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Keywords:
purification recombinant;
recombinant human;
sparc;
recombinant sparc ... See more keywords
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Published in 2019 at "Protein Expression and Purification"
DOI: 10.1016/j.pep.2019.105524
Abstract: Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of…
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Keywords:
purification recombinant;
protein coupled;
expression;
recombinant protein ... See more keywords