Discovery of a Novel Inhibitor of Human Purine Nucleoside Phosphorylase by a Simple Hydrophilic Interaction Liquid Chromatography Enzymatic Assay
Sign Up to like & getrecommendations! Published in 2021 at "ChemMedChem"
DOI: 10.1002/cmdc.202000874
Abstract: Human purine nucleoside phosphorylase (HsPNP) belongs to the purine salvage pathway of nucleic acids. Genetic deficiency of this enzyme triggers apoptosis of activated T‐cells due to the accumulation of deoxyguanosine triphosphate (dGTP). Therefore, potential chemotherapeutic… read more here.
Keywords: purine nucleoside; human purine; discovery novel; interaction ... See more keywords
Purine nucleoside analogs plus rituximab are an effective treatment choice for hairy cell leukemia-variant
Sign Up to like & getrecommendations! Published in 2022 at "Annals of Hematology"
DOI: 10.1007/s00277-022-04795-x
Abstract: Both characteristics and optimal treatment strategy for hairy cell leukemia-variant (HCL-v) remain elusive due to its rarity. We retrospectively analyzed the clinical features of HCL-v and the efficacy of first-line treatment options in a large… read more here.
Keywords: leukemia variant; purine nucleoside; treatment; cell leukemia ... See more keywords
Synthesis, characterization and biological evaluation of purine nucleoside analogues
Sign Up to like & getrecommendations! Published in 2017 at "Tetrahedron Letters"
DOI: 10.1016/j.tetlet.2017.09.041
Abstract: We present a convenient route for the synthesis of C6-amino-C5’-N-cyclopropyl carboxamido-C2-alkynylated purine nucleoside analogues 11a-g via Sonogashira coupling reaction. The nine step synthesis is easy to perform, employing commercially available reagents. Compound 9 is used… read more here.
Keywords: purine nucleoside; characterization biological; biological evaluation; analogues 11a ... See more keywords
Origin of Enzymatic Kinetic Isotope Effects in Human Purine Nucleoside Phosphorylase
Sign Up to like & getrecommendations! Published in 2017 at "ACS Catalysis"
DOI: 10.1021/acscatal.7b04199
Abstract: Here we report a study of the effect of heavy isotope labeling on the reaction catalyzed by human purine nucleoside phosphorylase (hPNP) to elucidate the origin of its catalytic effect and of the enzymatic kinetic… read more here.
Keywords: purine nucleoside; human purine; isotope; enzymatic kinetic ... See more keywords
Single tryptophan Y160W mutant of homooligomeric E. coli purine nucleoside phosphorylase implies that dimers forming the hexamer are functionally not equivalent
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-90472-4
Abstract: E. coli purine nucleoside phosphorylase is a homohexamer, which structure, in the apo form, can be described as a trimer of dimers. Earlier studies suggested that ligand binding and kinetic properties are well described by… read more here.
Keywords: purine nucleoside; dimers forming; y160w mutant; coli purine ... See more keywords
The First Purine Nucleoside Phosphorylase Deficiency Patient Resembling IgA Deficiency and a Review of the Literature
Sign Up to like & getrecommendations! Published in 2019 at "Immunological Investigations"
DOI: 10.1080/08820139.2019.1570249
Abstract: ABSTRACT Purine nucleoside phosphorylase (PNP) deficiency is a rare autosomal recessive primary immunodeficiency disorder characterized by decreased numbers of T-cells, variable B-cell abnormalities, decreased amount of serum uric acid and PNP enzyme activity. The affected… read more here.
Keywords: purine nucleoside; pnp; recurrent infections; first purine ... See more keywords
Interactions of 2,6-substituted purines with purine nucleoside phosphorylase from Helicobacter pylori in solution and in the crystal, and the effects of these compounds on cell cultures of this bacterium
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Enzyme Inhibition and Medicinal Chemistry"
DOI: 10.1080/14756366.2022.2061965
Abstract: Abstract Helicobacter pylori represents a global health threat with around 50% of the world population infected. Due to the increasing number of antibiotic-resistant strains, new strategies for eradication of H. pylori are needed. In this… read more here.
Keywords: nucleoside phosphorylase; substituted purines; interactions substituted; purine nucleoside ... See more keywords
Crystallization and Preliminary X-ray Diffraction Study of Purine Nucleoside Phosphorylase from the Thermophilic Bacterium Thermus thermophilus Strain HB27
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DOI: 10.1134/s1063774518050279
Abstract: Recombinant purine nucleoside phosphorylase from the thermophilic Thermus thermophilus strain encoded by the TT_C0194 gene was purified to homogeneity. The crystallization conditions for the enzyme were found by the vapor-diffusion technique. The crystals of the… read more here.
Keywords: ray diffraction; purine nucleoside; nucleoside phosphorylase;
Purine nucleoside phosphorylase inhibition ameliorates age-associated lower urinary tract dysfunctions
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DOI: 10.1172/jci.insight.140109
Abstract: In the aging population, lower urinary tract (LUT) dysfunction is common and often leads to storage and voiding difficulties classified into overlapping symptom syndromes. Despite prevalence and consequences of these syndromes, LUT disorders continue to… read more here.
Keywords: urinary tract; purine nucleoside; age; age associated ... See more keywords
Synthesis of New 5′-Norcarbocyclic Aza/Deaza Purine Fleximers - Noncompetitive Inhibitors of E.coli Purine Nucleoside Phosphorylase
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.867587
Abstract: A new series of flexible 5′-norcarbocyclic aza/deaza-purine nucleoside analogs were synthesized from 6-oxybicyclo[3.1.0.]hex-2-ene and pyrazole-containing fleximer analogs of heterocyclic bases using the Trost procedure. The compounds were evaluated as potential inhibitors of E. coli purine… read more here.
Keywords: norcarbocyclic aza; aza deaza; coli purine; deaza purine ... See more keywords
Radical Dehalogenation and Purine Nucleoside Phosphorylase E. coli: How Does an Admixture of 2′,3′-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis
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DOI: 10.3390/biom11040539
Abstract: During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3′-deoxyinosine catalyzed by E. coli purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered. An unknown nucleoside was… read more here.
Keywords: purine nucleoside; radical dehalogenation; admixture anhydroinosine; synthesis ... See more keywords