Towards water-free biobanks: long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells
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DOI: 10.1038/s41598-017-06945-y
Abstract: Desiccation-tolerant cultured cells Pv11 derived from the anhydrobiotic midge embryo endure complete desiccation in an ametabolic state and resume their metabolism after rehydration. These features led us to develop a novel dry preservation technology for… read more here.
Keywords: desiccation; dry preservation; enzyme; pv11 cells ... See more keywords
Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells
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DOI: 10.1038/s41598-021-98905-w
Abstract: Pv11 is an insect cell line established from the midge Polypedilum vanderplanki, whose larval form exhibits an extreme desiccation tolerance known as anhydrobiosis. Pv11 itself is also capable of anhydrobiosis, which is induced by trehalose… read more here.
Keywords: genome editing; signaling pathways; anhydrobiosis pv11; pv11 cells ... See more keywords
Intracellular Localization and Gene Expression Analysis Provides New Insights on LEA Proteins’ Diversity in Anhydrobiotic Cell Line
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DOI: 10.3390/biology11040487
Abstract: Simple Summary Polypedilum vanderplanki (sleeping chironomid) is widely known for its ability to withstand complete desiccation in a state of anhydrobiosis. The genome of this insect contains a number of hugely expanded paralogous gene groups,… read more here.
Keywords: localization; cell line; pvlea; terminal fusions ... See more keywords